| 张帅赛,丁易钤,刘春花.内皮脂肪酶rs3829632多态性与江苏地区宫颈癌易感性的关联关系及机制研究[J].,2018,(23):4552-4555 |
| 内皮脂肪酶rs3829632多态性与江苏地区宫颈癌易感性的关联关系及机制研究 |
| The Association of Endothelial Lipase rs3829632 Polymorphism with Cervical Cancer Susceptibility in Jiangsu Province and Its Mechanism |
| 投稿时间：2018-05-16 修订日期：2018-06-11 |
| DOI：10.13241/j.cnki.pmb.2018.23.036 |
| 中文关键词: 子宫颈癌 内皮脂肪酶 单核苷酸序列多态性 启动子 |
| 英文关键词: Cervical cancer Endothelial lipase Single nucleotide polymorphism Promoter |
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| 中文摘要: |
| 摘要 目的：探讨内皮脂肪酶（endothelial lipase, EL）rs3829632多态性对江苏地区女性宫颈癌易感性的影响。方法：采用病例-对照研究收集南通市妇幼保健院和南通市肿瘤医院宫颈癌病人328例及年龄匹配的对照人群356例，采集血标本提取基因组DNA运用DNA测序法对rs3829632位点进行基因分型；提取宫颈癌病理组织的mRNA及蛋白运用实时荧光定量PCR及Western blot检测其中EL的表达水平。结果：与基因型为rs3829632 TT的个体相比，携带rs3829632 C等位的个体宫颈癌患病风险显著升高；在宫颈癌病人中，rs3829632 CC基因型个体病理组织中EL的表达水平显著高于其他基因型个体。结论：EL rs3829632 C等位携带者其宫颈癌患病风险性升高，这一结果可能与发生在启动子区域的rs3829632影响了EL的表达及功能进而改变了宫颈细胞的脂质代谢状态有关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the association of EL rs3829632 polymorphism with cervical cancer in Jiangsu province. Methods: Using a case-control study, 328 CC patients and 356 age matched controls were enrolled in Nantong Maternal and Child Health Care Hospital and Nantong Cancer Hospital. Genomic DNA was extracted from blood samples and DNA sequence was used to determine the genotype of EL rs3829632 polymorphism. Real-time PCR and Western blot were used to determine the mRNA and protein expressions of EL in diseased tissues from cervical cancer patients. Results: Compare to TT genotype, individuals with rs3829632 C allele showed an increased risk for cervical cancer. And in cervical cancer patients, rs3829632 TT genotype carriers exhibited the highest EL expression both in mRNA and protein levels than other genotype carriers. Conclusion: The rs3829632 C allele is associated with increased risk for cervical cancer, which may be caused by abnormal expression and dysfunction of EL and subsequent aberrant lipid metabolism in cervical cells. |
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