| 纪奇峰,祝春来,王 伟,毛 壮,汪 钦,成 珊,颜 真.肝细胞癌患者血清外泌体microRNA表达鉴定[J].,2018,(22):4213-4218 |
| 肝细胞癌患者血清外泌体microRNA表达鉴定 |
| Exosomal MicroRNA Expression Identification in Hepatocellular Carcinoma Patients Serum |
| 投稿时间：2018-03-23 修订日期：2018-04-18 |
| DOI：10.13241/j.cnki.pmb.2018.22.003 |
| 中文关键词: 肝细胞癌 外泌体 miRNAs 肿瘤标志物 |
| 英文关键词: Hepatocellular carcinoma Exosome miRNAs Tumor biomarker |
| 基金项目:肿瘤生物学国家重点实验室自由探索项目（CBSKL2015Z14）；陕西省科技统筹创新工程项目（2016KTCL03-09） |
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| 中文摘要: |
| 摘要 目的：探讨肝癌细胞外泌体中差异表达的microRNAs(miRNAs)在肝细胞癌（HCC）诊断中的应用价值。方法：通过高通量测序筛选肝癌细胞外泌体中差异表达的miRNAs。实时定量PCR验证差异表达分子；检测差异表达的miRNAs在健康人（Health）、慢性乙型肝炎患者（CHB）、肝硬化患者（LC）及乙型肝炎病毒阳性的肝细胞癌患者（HCC）血清外泌体中的表达。结果：高通量测序筛选到肝癌细胞外泌体中差异表达的miRNA共88种，其中58种表达上调，30种表达下调。选择其中8种差异表达的miRNAs进行qRT-PCR验证，结果显示，此8种miRNAs在细胞上清外泌体、细胞内、癌与癌旁组织中的表达趋势与测序结果一致。miR-221-3p和miR-224-5p在HCC组外泌体中的表达水平显著高于Health组、CHB组和LC组(P<0.01)，miR-124-3p和let-7a-5p在HCC组外泌体中的表达水平显著低于其他各组(P<0.05)。四个组中，miR-21-5p、miR-191-5p、miR-34a-5p和miR-122-5p的表达水平不存在显著性差异（P>0.05）。结论：血清外泌体中的miR-221-3p、miR-224-5p、miR-124-3p 和let-7a-5p可能成为肝细胞癌的候选标志物。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the possible clinical application of differentially expressed exosomal miRNAs in hepatocellular carcinoma (HCC). Methods: The differential expressed miRNAs from HCC cells exosomes were screened by Next Generation Sequenc- ing. The expression levels of differentially expressed miRNAs were confirmed by quantitative real-time PCR and evaluated in healthy controls (Health), hepatitis B patients (CHB), liver cirrhosis patients (LC) and hepatitis B positive HCC patients. Results: Eighty-eight differentially expressed miRNAs were identified in exosomes of HCC cell lines through highthroughput sequencing, including 58 up-reg- ulated and 30 down-regulated miRNAs. Eight differentially expressed miRNAs were verified by qRT-PCR. It showed that miRNA ex- pression was consistent with the sequencing results in either cell line exosomes or in cell lines, also in tumor tissues vs. adjacent tissues. The expression levels of miR-221-3p and miR-224-5p in exosomes were significantly higher in HCC patients than those in Health, CHB or LC group(P<0.01). Furthermore, the expression levels of exosomal miR-124-3p and let-7a-5p were lower in HCC than Health, CHB or LC group(P<0.05). There was no significant difference in the expression of miR-21-5p, miR-191-5p, miR-34a-5p and miR-122-5p among the four groups(P>0.05). Conclusion: Serum exosomal miR-221-3p, miR-224-5p, miR-124-3p and let-7a-5p have the possibility to become canditate biomarkers for hepatocellular carcinoma diagnosis. |
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