| 陈利侠,张 汝,苏敏君,席晓薇,孙云燕.TRPM3通过Wnt/β-catenin信号通路诱导上皮性卵巢癌细胞上皮间质转化的作用研究[J].,2018,(22):4201-4206 |
| TRPM3通过Wnt/β-catenin信号通路诱导上皮性卵巢癌细胞上皮间质转化的作用研究 |
| TRPM3 induces Epithelial-mesenchymal Transition via the Wnt/β-catenin Signaling Pathway in Epithelial Ovarian Cancer |
| 投稿时间：2018-05-29 修订日期：2018-07-06 |
| DOI：10.13241/j.cnki.pmb.2018.22.001 |
| 中文关键词: 卵巢癌 上皮间质转化 瞬时受体电位离子通道3 |
| 英文关键词: Ovarian cancer Epithelial mesenchymal transition Transient receptor potential melastatin 3 |
| 基金项目:上海市自然科学基金项目(12ZR1424300) |
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| 中文摘要: |
| 摘要 目的：探讨瞬时受体电位离子通道3(Transient receptor potential melastatin 3，TRPM3)对卵巢癌侵袭转移和上皮细胞间质转化(Epithelial mesenchymal transition，EMT)的影响及其分子作用机制。方法：采用小干扰RNA沉默上皮性卵巢癌细胞株中HEY及SKOV3中TRPM3的表达，通过Transwell实验和划痕实验检测上皮性卵巢癌细胞的侵袭和迁移能力的变化，Western Blot检测EMT相关蛋白、Wnt/β-catenin通路相关蛋白的表达情况。结果：与对照组细胞相比，干扰组的上皮性卵巢癌细胞迁移和侵袭能力均明显减弱，EMT相关蛋白的上皮细胞标志分子E-cadherin的表达上调，间质细胞标志分子N-cadherin和EMT相关转录调控因子Snail的表达下调，Wnt/β-catenin通路相关蛋白CyclinD1、β-catenin的表达下调。结论：TRPM3可能通过激活Wnt/β-catenin通路促进卵巢癌细胞的上皮间质转化过程，进而增强其侵袭转移的能力。 |
| 英文摘要: |
| ABSTRACT Objective: The purpose of this study was to explore the role of TRPM3(transient receptor potential melastatin 3, TRPM3) in the invasion and metastasis of ovarian cancer, its role in EMT(epithelial mesenchymal transition, EMT), and the molecular mechanism in epithelial ovarian cancer. Methods: We knocked down expressionin of TRPM3 in HEY and SKOV3 cells, using a small interference RNA. Using Transwell experiment and scratche migration assays, we tried to detect the invasion and migration of epithelial ovarian cancer cells. In addition, the expression of related proteins in EMT and Wnt/β-catenin pathway was detected by Western Blot. Results: TRPM3 silencing increased the expression of E-cadherin and decreased the expression of N-cadherin, β-catenin and Snail. Conclusion: These results suggest that TRPM3 may enhance the ability of the invasion and metastasis of ovarian cancer cells by activating the Wnt/β-catenin pathway. |
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