| 文 娅,李 贝,邓雪松,林云涛,陆菁潇,吕国庆.α-硫辛酸下调Grb2抑制A549细胞增殖的研究[J].,2018,(21):4012-4016 |
| α-硫辛酸下调Grb2抑制A549细胞增殖的研究 |
| Alpha Lipoic Acid Inhibits A549 Cell Proliferation through the Repression of Grb2 |
| 投稿时间：2018-05-28 修订日期：2018-06-24 |
| DOI：10.13241/j.cnki.pmb.2018.21.003 |
| 中文关键词: α-硫辛酸 非小细胞肺癌 细胞增殖 Grb2 |
| 英文关键词: Alpha lipoic acid Non-small cell lung cancer Cell proliferation Growth factor receptor-bound protein 2 |
| 基金项目:国家自然科学基金面上项目（81672813）；人事部中国博士后科学基金项目（2016M602594）；深圳市科技计划2016年基础研究项目（JCYJ20160425105945739）；深圳市"三名工程"（SZSM201612051） |
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| 中文摘要: |
| 摘要 目的：探讨α-LA对A549细胞增殖的机制以及Grb2在其中发挥的作用。方法：利用CCK-8和流式细胞术检测α-LA对细胞增殖的影响；实时定量PCR和Western Blot检测细胞中Grb2表达的变化；利用向细胞中转染siGrb2及过表达Grb2载体检测细胞中Grb2表达水平差异对细胞增殖的影响。结果：α-LA处理A549 24 h后，对照组和α-LA处理组中位于G0/G1期细胞的比率由40.60％上升至57.80％；而位于S期细胞的比率由45.96％下降至39.01%；与细胞G1/S期的转换相关的调节蛋白CDK2/4/6, cyclin D3 和E1的表达也随之发生了相应的改变，α-LA通过抑制细胞G1/S期的转换而抑制细胞增殖。与亲本细胞相比，Grb2在α-LA处理A549细胞中的转录和蛋白表达水平均显著降低，干扰细胞中Grb2表达能显著抑制A549增殖；而过表达Grb2可阻断α-LA诱导的细胞增殖抑制。结论：α-LA具有抑制A549细胞增殖的作用，Grb2是α-LA发挥抑细胞增殖效应的重要介导因子。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the roles of growth factor receptor-bound protein 2 (Grb2) in Alpha lipoic acid inhibits cell pro- liferation process. Methods: The CCK-8 assay and flow cytometry were used to assess cell proliferation in A549 cell lines after α-LA treatment. The expression of Grb2, cyclin-dependent kinase 2(CDK2), CDK4, CDK6, Cyclin D3, Cyclin E1 was measured by western blotting. Grb2 levels were restored in α-LA-treated cells by transfection of a plasmid carrying Grb2 and were reduced in A549 cells via specific siRNA knockdown. Results: CCK8 assay and flow cytometry were shown that α-LA dramatically decreased A549 cell prolifera- tion. In control cells cultured without α-LA, the cells in the G1 and S phases were 40.60% and 45.96%, respectively. In α-LA treated group, the cells in the G1 and S phases were 57.80% and 39.01%, respectively, at 24 h Western Blot analysis indicated that α-LA de- creased the levels of CDK2/4/6, cyclin D3 and E1, events that were associated with the inhibition of the G1/S-phase transition. The level of Grb2 was downregulating in α-LA treated cells; in contrast, Grb2 overexpression significantly prevented α-LA-induced decreases in cell growth in vitro. Conclusion: These findings provide the first evidence that α-LA could inhibit cell proliferation and Grb2 is involved in this process. |
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