| 刘凤霞,李华强,斯依提·阿木提,艾孜孜·热合曼,侯慧慧,艾合麦提·玛木提,何嘉炜,夏丽丹·玉山,阿地力江·伊明.一种环境激素联合冷应激大鼠模型阴茎组织中 Sprouty 2、ERK1/2表达的研究[J].,2018,(18):3431-3434 |
| 一种环境激素联合冷应激大鼠模型阴茎组织中 Sprouty 2、ERK1/2表达的研究 |
| The Sprouty 2 and ERK1/2 Expression in the Penile Tissue of Rats Model Induced by Environmental Hormones Combined with Cold Stress |
| 投稿时间：2018-03-06 修订日期：2018-03-28 |
| DOI：10.13241/j.cnki.pmb.2018.18.006 |
| 中文关键词: 环境激素 冷应激 Sprouty 2 细胞外调节蛋白激酶 伊木萨克片 |
| 英文关键词: Environmental hormones Cold stress Sprouty 2 Extracellular regulated protein kinases1/2 Yimusake |
| 基金项目:国家自然科学基金联合基金项目（U1303322）；大学生创新性实验计划项目（CX2016074） |
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| 中文摘要: |
| 摘要 目的：研究 Sprouty 2（Spry 2）和细胞外调节蛋白激酶（ERK1/2）在环境激素联合冷应激大鼠模型阴茎组织中的表达，并观察伊木萨克片对二者表达的影响。方法：选用 30只正常的雄性 SD大鼠，其中 10只为正常对照组（N组），余 20只为造模组，采用富含环境雌激素饲料 +寒冷环境的干预条件 立复合性应激大鼠模型（20 w），其中 10只大鼠采用伊木萨克片干预 2 w（Y组），剩余 10只为模型组（M组）。采用免疫组化方法检测各组大鼠阴茎组织中 Spry 2、ERK1/2的表达。结果：M组大鼠阴茎组织中 Spry 2表达低于 N组（P<0.05），Y组大鼠阴茎组织中 Spry 2表达明显高于 M组（P<0.05）。三组大鼠阴茎组织中总 ERK1/2（t-ERK1/2）的表达比较差异均无统计学意义(P＞0.05)。M组大鼠阴茎组织中磷酸化 ERK1/2（p-ERK1/2）表达高于 N组（P<0.05），Y组大鼠阴茎组织中 p-ERK1/2表达明显低于 M组（P<0.05）。结论：Spry 2表达下调和 ERK1/2活化可能促进应激反应的发生发展，伊木萨克片可能通过上调 Spry 2的表达和减少 ERK1/2的活化发挥抗应激作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the Sprouty 2 (Spry 2) and extracellular regulated protein kinases1/2 (ERK1/2) expression in the penile tissue of rats model induced by environmental hormones and cold stress, and observe the effect of Yimusake. Methods:A total of 30 mature male Sprague-Dawley (SD) rats were randomly divided into 2 groups: normal control group (N group, n =10) and model group (n=20), the model rats were treated with environmental estrogen-like diet and cold stress for 20 weeks until establishment of stress rats model. Then the rats were randomly divided into 2 groups: stress model group (M group, n=10) and Yimusake intervention group (Y group, n=10), intervented for 2 weeks, the Spry 2 and ERK1/2 expressions were detected by immunohistochemistry. Results:(1) The Spry 2 expression in penile tissue of rats: M group was decreased compared with group N (P<0.05), Y group was increased compared with M group (P<0.05). (2) The total ERK1/2 (t-ERK1/2) expression in penile tissue had no statistical difference among three groups (P＞0.05). (3) Phosphorylated ERK1/2 (p-ERK1/2) expression in penile tissue: B1 group was obviously higher compared with N group (P<0.05); B3 group was lower compared with M group (P<0.05). Conclusion:Downregulation of Spry 2 expression and activity of ERK1/2 can enhance the development of sress response, Yimusake play a anti-stress role by upregulating the Spry 2 expression and inhibiting the activation of ERK1/2. |
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