| 王海玮,柴丽丽,杨国青,司望利,陈 燕.胃癌患者外周血调节性T细胞水平与免疫抑制状态及病理特征的关系研究[J].,2018,(17):3365-3369 |
| 胃癌患者外周血调节性T细胞水平与免疫抑制状态及病理特征的关系研究 |
| Research on Relationship Between Regulatory T Cell Levels in Peripheral Blood and Immunosuppressive Status and Pathological Features in the Patients with Gastric Cancer |
| 投稿时间：2017-10-29 修订日期：2017-11-24 |
| DOI：10.13241/j.cnki.pmb.2018.17.036 |
| 中文关键词: 胃癌 调节性T细胞 免疫抑制状态 相关性 |
| 英文关键词: Gastric cancer Regulatory T cell Immunological inhibition status Correlation |
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| 中文摘要: |
| 摘要 目的：探讨胃癌患者外周血中CD4+CD25+调节性T细胞（Treg）水平与免疫抑制状态和病理特征的关系。方法：选择2016年1月至2017年6月我院收治的胃癌患者73例作为胃癌组，另选同期在本院进行体格检查的健康者41例作为对照组，采用流式细胞仪检测其外周血中CD4+CD25+Treg水平，采用酶联免疫吸附试验（ELISA）检测血清γ-干扰素（IFN-γ）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-10（IL-10）水平，分析CD4+CD25+Treg水平与IFN-γ、IL-2、IL-4和IL-10的相关性及与病理特征的关系。结果：胃癌组CD4+CD25+Treg比例、IL-4和IL-10水平为（19.43±4.36）%、（9.76±2.41）pg/mL和（22.18±5.26）pg/mL，高于对照组的（10.34±2.16）%、（7.16±2.07）pg/mL和（9.52±3.47）pg/mL，差异有统计学意义（P＜0.05）；胃癌组IFN-γ和IL-2水平为（6.87±2.24）pg/mL和（2.43±0.54）pg/mL，低于对照组的（13.86±3.18）pg/mL和（12.79±2.16）pg/mL，差异有统计学意义（P＜0.05）。CD4+CD25+Treg比例与IFN-γ和IL-2呈负相关关系（P＜0.05），与IL-4和IL-10呈正相关关系（P＜0.05）。CD4+CD25+Treg比例与胃癌患者的TNM分期、淋巴结转移有关（P＜0.05），与病理类型、肿瘤直径、分化程度和肿瘤位置无关（P＞0.05）。结论：胃癌患者外周血Treg水平增高，与免疫状态有明显的相关性，其参与了肿瘤的发生与发展过程。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the levels of CD4+CD25+ regulatory T cells (Treg) in peripheral blood of patients with gastric cancer and the immunosuppressive state and pathological features. Methods: 73 patients with gastric cancer who were treated in our hospital from January 2016 to June 2017 were selected as gastric cancer group, 41 healthy persons who under- went physical examination in our hospital were selected as control group in the same period.The levels of CD4+CD25+Treg in peripheral blood were detected by flow cytometry,serum levels of interferon-γ(IFN-γ), interleukin-2 (IL-2), interleukin -4 (IL-4) and interleukin -10 (IL-10) were measured by enzyme linked immunosorbent assay(ELISA), the correlation between CD4+CD25+Treg level and IFN-γ, IL-2, IL-4 and IL-10 and the relationship with the pathological features were analyzed. Results: The ratio of CD4+CD25+Treg, the levels of IL-4 and IL-10 in the gastric cancer group were (19.43±4.36)%, (9.76±2.41)pg/mL and (22.18±5.26)pg/mL, which were higher than (10.34±2.16)%, (7.16±2.07)pg/mL and (9.52±3.47)pg/mL of the control group, the differences were statistically significant(P<0.05). The level of IFN-γ and IL-2 in gastric cancer group were (6.87±2.24)pg/mL and (2.43±0.54)pg/mL, which were lower than(13.86±3.18)pg/mL and (12.79±2.16)pg/ml of the control group,the differences were statistically significant(P<0.05). The proportion of CD4+CD25+Treg was negatively correlated with IFN-γ and IL-2(P<0.05), and there was a positive correlation with IL-4 and IL-10(P<0.05). The ratio of CD4+CD25+Treg was related to TNM stage and lymph node metastasis in gastric cancer patients (P<0.05), which was in- dependent of pathological type, tumor diameter, differentiation degree and tumor location (P>0.05). Conclusion: The level of Treg in pe- ripheral blood of patients with gastric cancer is higher, and it is closely related to the immune status, which is involved in the process of tumor develop- ment. |
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