| 刘佐君,张树菊,孟凡彪,钱民先,敖 英.亚麻酸对肝癌细胞HepG2节律的调控研究[J].,2018,(16):3028-3031 |
| 亚麻酸对肝癌细胞HepG2节律的调控研究 |
| Effects of Alpha-Linolenic Acid on the Circadian Expression Pattern of Human Liver Carcinoma HepG2 Cells |
| 投稿时间：2017-11-28 修订日期：2017-12-23 |
| DOI：10.13241/j.cnki.pmb.2018.16.006 |
| 中文关键词: 节律钟 亚麻酸 脂肪酸合成 免疫促炎因子 |
| 英文关键词: Circadian clock Alpha-Linolenic acid Fatty acid biosynthesis Proinflammatory cytokine |
| 基金项目:国家自然科学基金项目（81601215）；深圳市科技创新基础研究项目（JCYJ20150525092941002） |
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| 中文摘要: |
| 摘要 目的：探讨多不饱和脂肪酸亚麻酸对肝癌细胞HepG2节律钟的影响。方法：通过50%的马血清刺激诱导HepG2细胞同步化，利用亚麻酸处理同步化后的HepG2细胞。进一步利用荧光定量PCR和western-blot检测节律钟关键基因的变化。结果：HepG2细胞经过亚麻酸处理后节律钟关键基因芳烃受体核转位蛋白3（brain and muscle Arnt-like protein-1，BMAL1）和蓝光受体蛋白1（Cryptochrome 1，CRY1）在转录水平的表达水平有所降低。与此一致的是，在蛋白质水平CRY1和BMAL1的表达同样受到亚麻酸的抑制。同时发现，CRY1的转录水平的节律周期有明显的缩短。进一步研究发现， 亚麻酸对HepG2细胞的脂肪酸合成关键基因脂肪酸合酶（Fatty acid synthase，FASN）和硬脂酰辅酶A脱氢酶(Stearoyl-CoA desaturase 1，SCD1),以及免疫促炎因子白细胞介素-6（Interleukelin-6，IL-6）和白细胞介素-8（Interleukelin-8，IL-8）的mRNA的表达具有明显的抑制效应。结论：亚麻酸影响了肝癌HepG2细胞水平的节律基因的表达水平以及缩短了节律基因的周期，并且对于HepG2细胞的脂肪酸合成以及免疫促炎因子有明显的抑制效应。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of alpha-Linolenic acid on the cell autonomous circadian rhythm of HepG2 cells. Methods: The human liver carcinoma HepG2 cells were synchronized by 50% horse serum, and treated with BSA or 200 μmol/L alpha-Linolenic acid. The oscillation profiles of circadian genes were examined by qRT-PCR and western blot. Results: alpha-Linolenic acid slightly inhibited the amplitude of the mRNA levels of several key clock components, including CRY1 and BMAL1. The circadian period length of CRY1 were reduced by alpha-Linolenic acid treatment. Western blot analysis revealed alpha-Linolenic acid treatment led to reduced protein levels of CRY1 and BMAL1. In addition, alpha-Linolenic acid dramatically inhibited oscillation of fatty acid synthesis (FASN and SCD1) and proinflammatory cytokines (IL-6 and IL-8). Conclusion: alpha-Linolenic acid inhibited amplitude of several circadian core clock genes, and reduced period length of CRY1. Meanwhile, alpha-Linolenic acid strongly inhibited expression of fatty acid synthesis and proinflammatory cytokines. |
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