| 王 彤,崔江波,王 芳,宋雅娟,马显杰,苏映军,彭 湃,余 州.不同移植部位对成年大鼠睾丸间质细胞雄激素分泌功能的影响[J].,2018,(13):2414-2418 |
| 不同移植部位对成年大鼠睾丸间质细胞雄激素分泌功能的影响 |
| The Effects of Different Transplant Sites on Androgen Secretion of Adult Rat Leydig Cells |
| 投稿时间：2018-01-05 修订日期：2018-02-08 |
| DOI：10.13241/j.cnki.pmb.2018.13.003 |
| 中文关键词: 睾丸移植 移植部位 睾丸间质细胞 雄激素 分泌 |
| 英文关键词: Testis transplantation Transplant site Leydig cell Androgen Secretion |
| 基金项目:国家自然科学基金项目(30973752,81272118)；西京医院学科助推计划项目（XJZT15M23） |
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| 中文摘要: |
| 摘要 目的：探索不同移植部位对移植的成年SD大鼠睾丸中睾丸间质细胞存活及雄激素分泌功能的影响。方法：将健康成年雄性SD大鼠随机分为对照组、假手术组、皮下组和肾包膜组。对照组大鼠不去势，其余大鼠于睾丸移植前1周行去势手术。对照组和假手术组去势后仅行背部皮肤切开，不进行睾丸移植；皮下组背部两侧各移植1/3个成年SD大鼠睾丸组织；肾包膜组每侧肾包膜下移植1/3个成年SD大鼠睾丸组织。4周后取材行HE和免疫组化染色，分析移植睾丸组织中睾丸间质细胞存活情况，ELISA法检测受体大鼠血清睾酮水平。结果：皮下组和肾包膜组移植物中难于见到完整的睾丸间质组织，但免疫组化染色发现大量HSD-17β1阳性细胞，对照组、皮下组和肾包膜组的HSD-17β1阳性细胞数分别为（24.33±4.30）、（9.83±4.05）和（12.67±2.81）个，对照组与皮下组相比差异具有统计学意义（p<0.05）；ELISA分析发现对照组、假手术组、皮下组和肾包膜组的血清睾酮浓度分别为（3.81±1.32）、（0.28±0.08）、（0.44±0.13）和（0.90±0.31）ng/mL，肾包膜组血清睾酮浓度高于假手术组（p<0.01）和皮下组（p<0.05），而皮下组血清睾酮水平高于假手术组，但两者差异无统计学意义（p>0.05）。结论：移植的成年大鼠睾丸组织中的睾丸间质细胞可在受体肾包膜下或皮下存活，但肾包膜下移植可能更加有利于睾丸间质细胞存活和雄激素分泌。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effects of different transplant sites on survivaland androgen section of Leydig cell of adult rat testis. Methods: Healthy adult rats were randomly divided into control group, sham group, subcutaneous group, and kidney capsule group. Rats in control groupkept intact, the remaining rats underwent castration 1 week before testis transplantation. The dorsal skin of rats in control group and sham group were cut, but no testiswastransplanted subcutaneously; 1/3 testis was transplanted subcutaneously at each side of dorsal skin of rats in subcutaneous group, while testis of the same volume was transplanted under the kidney capsule of rats in the kidney capsule group. The grafts were obtained 4 weeks later. Then the Leydig cell were examined by using HE staining and immunohistochemistry staining. And the testosterone concentration of recipient serum was analyzed by ELISA. Results: Interstitial tissues intact were not found in grafts in subcutaneous group and kidney capsule group. Butnumerous HSD-17β1-positive cells were observed in samples of both groups. And the number of HSD-17β1-positive cell of control group, subcutaneous group, and kidney capsule were(3.81±1.32), (9.83±4.05), and(12.67±2.81) separately. The testosterone concentration of rat serum of control group, sham group, subcutaneous group, and kidney capsule group were(3.81±1.32), (0.28±0.08), (0.44±0.13), and (0.90±0.31)ng/mL separately. The testosterone concentration of kidney capsule group was higher than that in sham group(p<0.01) and subcutaneous group (p<0.05), while the testosterone concentration of serum in subcutaneous group was higher than that in sham group, but the difference between the two groups was not statistically significant (p>0.05). Conclusion: Leydig cells in testis grafts could survive under the kidney capsule or back skin of recipients. However, the microenvironment of under kidney capsule might be more suitable for Leydig cell survival and androgen secretion. |
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