| 闫汝虎,李 楠,张二飞,涂 可,阴弯弯,张 莉,杜诗斌,侯立朝,孙冬冬.IFITM3在脓毒症模型及胆碱能抗炎模型中的表达[J].,2018,(12):2257-2260 |
| IFITM3在脓毒症模型及胆碱能抗炎模型中的表达 |
| Expression of IFITM3 in Sepsis and Cholinergic Anti-inflammatory Pathway |
| 投稿时间：2018-01-10 修订日期：2018-02-07 |
| DOI：10.13241/j.cnki.pmb.2018.12.012 |
| 中文关键词: IFITM3 胆碱能抗炎通路 脓毒症 |
| 英文关键词: IFITM3 Cholinergic anti-inflammatory pathway Sepsis |
| 基金项目:国家自然科学基金项目（81570318） |
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| 中文摘要: |
| 摘要 目的：探讨干扰素诱导的跨膜转运蛋白3（IFITM3）在LPS刺激的RAW264.7细胞系的脓毒症模型中的表达以及胆碱能抗炎模型中的表达。方法：用1 μg/mL LPS刺激RAW264.7细胞24、48、72 h后，用Western-Blot法检测各组细胞IFITM3蛋白表达水平。用1 μg/mL LPS刺激RAW264.7细胞后，给予50 μM胆碱能受体激动剂GTS-21以及同时给予100 nM胆碱能受体拮抗剂α-BGT刺激细胞24 h后,用Western-Blot法检测各组细胞IFITM3蛋白表达水平。用ELISA法检测IL-1?茁的方法验证脓毒症模型和胆碱能抗炎模型的建立。结果：①1 μg/mL LPS刺激RAW264.7细胞后，IFITM3蛋白表达明显降低（P<0.01）。 ②1 μg/mL LPS刺激RAW264.7细胞后再给予50 μM GTS-21，IFITM3蛋白表达明显升高（P<0.001）；而给予100 nM α-BGT后，IFITM3蛋白表达明显降低（P<0.001）。结论：LPS刺激的RAW264.7细胞IFITM3蛋白表达降低。给予胆碱能激动剂GTS-21后能够逆转LPS诱导的IFITM3表达的降低，给予胆碱能受体拮抗剂α-BGT则能阻断这种现象。IFITM3有可能在脓毒症中发挥保护作用，并且参与了胆碱能抗炎通路抗炎过程的调节。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of IFITM3 in RAW264.7 cell line stimulated by LPS and Cholinergic anti-in- flammatory pathway model. Methods: RAW264.7 cell was treated by 1 μg/mL LPS for 24, 48 and 72 h, the protein expression of IFITM3 was determined by Western Blot. 50 μM Cholinergic receptor agonist GTS-21 combined with 100 nM Cholinergic receptor an- tagonist α-BGT were given after 1 μg/mL LPS stimulation and then the protein expression levels of IFITM3 were determined. The model of sepsis and Cholinergic anti-inflammatory pathway were verified by IL-1β level detection. Results: ①The expression of IFITM3 in RAW264.7 cell lines were greatly decreased after 1 μg/mL LPS stimulation (P<0.01). ②The expression of IFITM3 was significantly in- creased after treatment with 50 μM GTS-21 for 24 h (P<0.001) but notably declined after treatment of 100 nM α-BGT for 24 h following 1 μg/mL LPS stimulation (P<0.001). Conclusion: The protein expression of IFITM3 was decreased in RAW264.7 cell induced by LPS. Cholinergic receptor agonist GTS-21 could reverse the reduction of IFITM3 induced by LPS, and this effect was abol- ished by Choliner- gic receptor antagonist α-BGT. IFITM3 might have protective effect in sepsis and regulate anti-inflammatory process in Cholinergic anti- inflammatory pathway. |
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