| 刘 云,甘 为,张正龙,陈 胜,王天宝,李云飞.miR-182在前列腺癌组织中的表达及其对前列腺癌细胞增殖和凋亡的影响[J].,2018,(9):1674-1678 |
| miR-182在前列腺癌组织中的表达及其对前列腺癌细胞增殖和凋亡的影响 |
| miR-182 Expression in the Prostate Cancer Tissue and its Effect on the Proliferation and Apoptosis of Prostate Cancer Cells |
| 投稿时间：2017-11-02 修订日期：2017-11-30 |
| DOI：10.13241/j.cnki.pmb.2018.09.015 |
| 中文关键词: 前列腺癌 miR-182 细胞增殖 细胞凋亡 |
| 英文关键词: Prostate cancer miR-182 Cell proliferation Apoptosis |
| 基金项目:湖北省卫生和计划生育委员会科研项目（WJ2015MB220） |
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| 中文摘要: |
| 摘要 目的：观察前列腺癌组织及不同前列腺癌细胞系中miR-182的表达，并探讨下调其表达对前列腺癌细胞增殖和凋亡的影响及机制。方法：采用实时荧光定量PCR(qRT-PCR)检测30例前列腺癌组织和30例相应的癌旁组织以及前列腺正常上皮RWPE-1细胞、前列腺癌PC-3、LNCaP和DU145细胞中miR-182的表达，进一步采用Lipfectamine 2000脂质体转染miRNA-182 inhibitor和阴性对照miRNA于PC-3细胞后，通过噻唑蓝(MTT)比色法检测细胞增殖情况，流式细胞术检测细胞凋亡率，免疫印迹(West- ern blot)法检测转录因子FOXO1、血管内皮生长因子(VEGF)和抑癌基因p53蛋白的表达。结果：miR-182在前列腺癌组织中的表达明显高于癌旁组织(P<0.05)；miR-182在前列腺癌细胞系PC-3、LNCaP和DU145中的表达均高于前列腺正常上皮细胞RW- PE-1(P<0.05)，其中PC-3细胞中miR-182表达水平最高。转染miRNA-182 inhibitor至PC-3细胞成功下调miR-182表达后，细胞的增殖能力明显受到抑制，细胞凋亡能力明显增强，FOXO1表达水平显著升高，VEGF和p53的表达明显降低，差异均具有统计学意义(P<0.05)。结论：miR-182在前列腺癌组织及细胞中呈高表达，下调miR-182的表达可能通过增加FOXO1的表达并减少VEGF和p53的表达，抑制前列腺癌细胞增殖并诱导细胞凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the expression of miR-182 in prostate cancer tissues and different prostate cancer cell lines, and investigate the effect and mechanism of down-regulation of miR-182 expression on the proliferation and apoptosis of prostate cancer cells. Methods: The expression of miR-182 in 30 cases of prostate cancer tissues, 30 cases of adjacent normal tissues, and RWPE-1 cells of prostate epithelium and PC-3, LNCaP and DU145 cells of prostate cancer were detected by real-time quantitative PCR (qRT-PCR). MiRNA-182 inhibitor and negative control miRNA were transfected with Lipfectamine 2000 liposome in PC-3 cells, and cell prolifera- tion was detected by MTT assay, apoptosis rate was detected by flow cytometry, and expression of transcription factor FOXO1, vascular endothelial growth factor (VEGF) and tumor suppressor gene p53 protein were detected by Western blot. Results: The expression of miR-182 in prostate cancer tissues was significantly higher than that in paracancerous tissues (P<0.05); The expressions of miR-182 in prostate cancer cell lines PC-3, LNCaP and DU145 were higher than that in normal prostate epithelial cells RWPE-1(P<0.05), in which PC-3 cells miR-182 expression level was the highest. Transfection of miRNA-182 inhibitor into PC-3 cells successfully downregulate miR-182 expression, and cell proliferation was inhibited, apoptosis capacity significantly was enhanced, and the expression level of FOXO1 was significantly increased, the expression of VEGF and p53 was decreased significantly, which differences were statistically significant (P<0.05). Conclusion: MiR-182 is highly expressed in prostate cancer tissues and cells, and down regulation of miR-182 expression may inhibit the proliferation and induce apoptosis of prostate cancer cells by increasing the expression of FOXO1 and decreasing the expres- sion of VEGF and p53. |
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