| 邵 菲,孙 姝,杨丹丹,高胜利,徐 珞.Orexin在隔核对大鼠胃传入信息的调控[J].,2018,(7):1264-1267 |
| Orexin在隔核对大鼠胃传入信息的调控 |
| Regulation of Orexin on the Gastric Afferent Information in Rats |
| 投稿时间：2017-06-09 修订日期：2017-06-30 |
| DOI：10.13241/j.cnki.pmb.2018.07.013 |
| 中文关键词: Orexin-A 隔核 胃传入信息 胃运动 |
| 英文关键词: Orexin-A Septal nucleus Gastric afferent information Gastric motility |
| 基金项目:国家自然科学基金项目（81470815,81270460,81500414）；山东省优秀中青年科学基金项目（BS2014YY009）；青岛市科技局项目（14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要 目的：研究orexin在隔核对大鼠胃传入信息的调控作用。方法：选取健康成年雄性Wistar大鼠138只(体质量250-300 g)，记录神经元放电活动，鉴定隔核胃牵张(GD)敏感性神经元；隔核微量注射orexin-A或orexin-A受体拮抗剂SB334867，观察隔核GD敏感性神经元放电活动变化；隔核微量注射不同浓度的orexin-A，观察大鼠胃运动的变化。结果：隔核微量注射orexin-A的大鼠胃运动幅度和频率显著增加，并呈剂量依赖关系(P<0.05-0.01)，微量注射SB-334867可完全阻断orexin-A对胃运动的影响。隔核微量注射orexin-A后，有36个GD-E神经元兴奋(P<0.01)，16个GD-I神经元抑制。Orexin-A受体拮抗剂SB334867可完全阻断orexin-A对GD敏感神经元的作用。结论：隔核注射orexin能促进大鼠胃运动，并影响胃牵张敏感神经元的放电活动。 |
| 英文摘要: |
| ABSTRACT Objective: To study the regulation of orexin on the gastric afferent information in the septal nuclei of rats. Methods: 138 adult male Wistar rats weighed 250-300 g were selected, extracellular discharges of single unit neuron in the septal nuclei were used to identify the gastric distension(GD) sensitive neurons. Microinjection of orexin-A or orexin-A receptor antagonist SB334867 into septal nuclei was performed to explore the changes of GD sensitive neurons in the septal nuclei. The effects of different concentrations of orex- in-A on the gastric motility of rats were observed. Results: The gastric motility experiments showed that administration of orexin-A in the septal nuclei could significantly increase the amplitude and frequency of gastric motility in a dose-dependent manner(P<0.05-0.01). Mi- croinjection of SB-334867 could completely block the effect of orexin-A on the gastric motility. After the septal nuclei Microinjection of orexin-A, there were 36 GD excitatory (GD-E) neurons and 16 GD inhibitory (GD-I) neurons. The effects induced by orexin-A on GD sensitive neurons were completely abolished with administration of SB-334867. Conclusion: Injection of orexin into the septal nuclei could promote the gastric motility in rats and also change the discharge activity of gastric distention (GD) sensitive neurons. |
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