| 万晓龙,赵 楠,景鹏瑞,鲁晓岚,董水滢,史海涛.miR-125a-3p调控人结肠癌细胞浸润转移的作用及机制研究[J].,2018,(7):1212-1216 |
| miR-125a-3p调控人结肠癌细胞浸润转移的作用及机制研究 |
| Effect and Mechanism of miR-125a-3p on the Infiltration and Metastasis of Human Colon Cancer Cells |
| 投稿时间：2017-08-17 修订日期：2017-08-31 |
| DOI：10.13241/j.cnki.pmb.2018.07.003 |
| 中文关键词: 结肠癌 肿瘤进展 转移 miR-125a-3p 上皮间质转化 |
| 英文关键词: Colorectal cancer Infiltration Metastasis miR-125a-3p Epithelial mesenchymal transition |
| 基金项目:陕西省科学技术研究发展计划项目（2016SF-009） |
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| 中文摘要: |
| 摘要 目的：探讨miR-125a-3p在结肠癌细胞浸润与转移中的作用及其可能机制。方法：通过qRT-PCR方法检测miR-125a-3p在结肠癌细胞及组织样本中的表达；在结肠癌细胞过表达或沉默miR-125a-3p后，通过平板克隆实验、MTT实验、划痕实验、Transwell实验检测结肠癌细胞增殖、迁移及侵袭能力的变化；采用Western blot方法检测miR-125a-3p过表达后相关标志分子的表达水平变化情况。结果：miR-125a-3p在结肠癌细胞及组织呈现异常低表达；过表达miR-125a-3p抑制结肠癌细胞HCT116及SW480的增殖能力；过表达或沉默miR-125a-3p分别抑制或增强结肠癌细胞的迁移与侵袭能力；过表达miR-125a-3p在mRNA及蛋白水平均能够显著抑制Snail、N-cadherin及Vimentin的表达，而增加E-cadherin的表达。结论：miR-125a-3p参与调节结肠癌细胞浸润与转移，其机制可能是通过调控上皮间质转化途径介导的。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect and mechanism of miR-125a-3p on the infiltration and metastasis of colon cancer cells. Methods: The expression of miR-125a-3p in colon cancer cells and tissue samples were detected by qRT-PCR. The miR-125a-3p expression in the colon cancer cells was overexpressed or down-regulated, then the effects of miR-125a-3p on the proliferation, migration and invasion of colon cancer cells were detected by colony formation assay, cell proliferation assay, wound healing assay and transwell arrays. The expressions of epithelial-mesenchymal transition(EMT) related markers were detected by Western blot after miR-125a-3p-overexpressed colon cancer cells. Results: The expression of miR-125a-3p was significantly down-regulated in both colon cancer cells and tissues. Overexpression of miR-125a-3p inhibited the proliferation of colon cancer cells HCT116 and SW480. Overex- pression or silence of miR-125a-3p inhibited or enhanced the migration and invasion of colon cancer cells respectively. In miR-125a-3p-overexpressed colon cancer cells, the expression of the Snail, N-cadherin and Vimentin were significantly decreased, whereas E-cadherin expression was increased in both mRNA and protein levels. Conclusion: miR-125a-3p participated in regulating the migration and invasion of colon cancer cells. The mechanism may be mediated by regulating the EMT pathway. |
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