| 谢鹏昆,栾 晓,柳 洋,郭菲菲,孙向荣,徐 珞.姜辣素对链霉素诱导糖尿病大鼠ghrelin表达及胃轻瘫的影响[J].,2018,(6):1067-1072 |
| 姜辣素对链霉素诱导糖尿病大鼠ghrelin表达及胃轻瘫的影响 |
| The Effect of Gingerol on the Expression of Ghrelin and Gastroparesis in Gastric Tissues of Streptozotocin-induced Diabetic Rats |
| 投稿时间：2017-04-30 修订日期：2017-05-26 |
| DOI：10.13241/j.cnki.pmb.2018.06.013 |
| 中文关键词: 姜辣素 糖尿病胃轻瘫 ghrelin 胃排空 氧化应激 |
| 英文关键词: Gingerol Diabetic gastroparesis Ghrelin Gastric emptying Oxidative stress |
| 基金项目:国家自然科学基金项目（81470815，81270460，81500414）；山东省优秀中青年科学基金项目（BS2014YY009）；青岛市科技局项目（14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要 目的：探究姜辣素对链霉素诱导糖尿病大鼠胃肠功能的影响。方法：链霉素诱导糖尿病大鼠连续灌胃姜辣素（100, 200, 400 mg/kg）28天，检测胃部超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、谷胱甘肽（GSH）和丙二醛（MDA）含量以及胃排空。结果：糖尿病大鼠SOD、CAT以及GSH水平降低（SOD: 6.29±1.03 vs. 3.41±1.21; CAT: 27.43±5.27 vs. 10.52±2.37, GSH: 1091.27±170.09 vs. 685.07±75.24，P＜0.05），MDA水平增高（9.79±2.41 vs. 46.38±12.59，P＜0.05），姜辣素灌胃后SOD、CAT以及GSH水平增高（SOD: 3.41±1.21 vs. 4.36±1.01, 5.62±1.18, 7.05±1.48, 6.48±1.82; CAT: 10.52±2.37 vs. 15.27±4.59, 19.29±5.42, 23.79±6.35, GSH: 10.52±2.37 vs. 15.27±4.59, 19.29±5.42, 23.79±6.35，P＜0.05-0.01），MDA水平降低（46.38±12.59 vs. 34.61±9.27, 28.01±8.34, 19.17±5.19, P＜0.05-0.01）；糖尿病大鼠胃ghrelin水平下降（381.26±94.37 vs. 195.07±57.42, P＜0.01）血浆ghrelin水平上升（76.86±21.81 vs. 108.83±27.75, P＜0.05）胃ghrelin mRNA表达增多，给予中高剂量姜辣素后胃ghrelin水平上升（195.07±57.42 vs. 301.43±81.24， 328.93±76.59，P＜0.05~0.01）胃ghrelin mRNA表达减少，呈量效依赖关系；糖尿病大鼠胃排空降低（82.24±19.74 vs. 45.37±11.23, P＜0.01），给予姜辣素后胃排空增强（45.37±11.23 vs. 52.43±15.42, 49.89±9.84, 74.39±20.79, P＜0.05-0.01）。结论：姜辣素通过抗氧化性改善糖尿病胃轻瘫。 |
| 英文摘要: |
| ABSTRACT Objective: Aim to investigate the effect of gingerol on gastrointestinal function in streptozotocin-induced diabetic rats. Methods: Gingerol was administrated intragastrically at a dose of 100, 200 and 400 mg/kg/day to diabetic rats. After 28 dayst, assayed the activity of superoxide dismutase (SOD), catalase (CAT), the content of reduced glutathione (GSH), malondialdehyde (MDA) in gastric mucosa and gastric emptying. Results: The diabetic rats exhibited significant decreases in the above mentioned antioxidative enzymes activities and GSH level and exhibited a high level of MDA（SOD: 6.29±1.03 vs. 3.41±1.21; CAT: 27.43±5.27 vs. 10.52±2.37, GSH: 1091.27±170.09 vs. 685.07±75.24，MDA: 9.79±2.41 vs. 46.38±12.59，P＜0.05）. After administration of gingerol, SOD, CAT and GSH level increase (SOD: 3.41±1.21 vs. 4.36±1.01, 5.62±1.18, 7.05±1.48, 6.48±1.82; CAT: 10.52±2.37 vs. 15.27±4.59, 19.29±5.42, 23.79±6.35, GSH: 10.52±2.37 vs. 15.27±4.59, 19.29±5.42, 23.79±6.35，P＜0.05-0.01）, MDA level decrease（46.38±12.59 vs. 34.61±9.27, 28.01±8.34, 19.17±5.19, P＜0.05-0.01）; After administration of gingerol, the parameters were ameliorated to a large extent; gingerol treatment dose-dependently augmented the ghrelin levels of stomach and plasma（195.07±57.42 vs. 301.43±81.24， 328.93±76.59, P＜0.05~0.01）, which were earlier depleted in the diabetic control rats; the expression of ghrelin mRNA was decreased after gingerol treatment; the gastric emptying in gingerol treated diabetic rats（45.37±11.23 vs. 52.43±15.42, 49.89±9.84, 74.39±20.79, P＜0.05-0.01） was notably accelerated compared with the diabetic control rats. Conclusion: The gingerol can against streptozotocin-induced gastroparesis possibly by its antioxidant property. |
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