| 刘 嵬,侯曦露,历海清,郭世存,余森源,高 倩,严和中,刘 卫,唐 郡.FPR2介导内外源性配体HP(2-20)/ANXA1促进胃癌细胞侵袭和转移[J].,2018,(6):1055-1059 |
| FPR2介导内外源性配体HP(2-20)/ANXA1促进胃癌细胞侵袭和转移 |
| Endogenous and Exogenous Ligands Promote the Invasion and Metastasis of Gastric Cancer Cells by FPR2 |
| 投稿时间：2017-10-18 修订日期：2017-11-26 |
| DOI：10.13241/j.cnki.pmb.2018.06.011 |
| 中文关键词: FPR2 配体 胃癌 侵袭转移 |
| 英文关键词: FPR2 Ligands Gastric cancer Invasion and metastasis |
| 基金项目:解放军南京军区医学科技创新经费资助项目（15MS046） |
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| 中文摘要: |
| 摘要 目的：分析FPR2在内外源性配体对胃癌细胞侵袭和转移影响中的作用。方法：采用构建成功的XN0422/SGC7901-mock细胞和XN0422/SGC7901-shFPR2低表达细胞，通过划痕实验、transwell实验观察和比较FPR2的内外源性配体－HP(2-20)/ANXA1对XN0422/SGC7901迁移和增殖的影响。结果：HP(2-20)/ANXA1处理的XN0422/SGC7901-mock和XN0422/SGC7901-shFPR2细胞侵袭和转移能力均相较于DMSO处理的XN0422/SGC7901-mock和XN0422/SGC7901-shFPR2细胞明显增强(P<0.05)，且 XN0422/SGC7901-mock组细胞的侵袭转移能力明显优于XN0422/SGC7901-shFPR2组细胞(P<0.05)。结论：HP2-20和Ac2-26可以促进胃癌细胞的侵袭和迁移，而这一作用与FPR2有关。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the effect of FPR2 on the invasion and metastasis of gastric cancer cells under the influence of endogenous and exogenous ligands. Methods: XN0422/SGC7901-mock cells and XN0422/SGC7901-shFPR2 cells previously constructed were used for the experiment. By using wound-healing assay and transwell assay, the effect of endogenous and exogenous ligands of FPR2-HP(2-20)/ANXA1 on the invasion and metastasis of XN0422/SGC7901 cells were compared. Results: The ability of invasion and metastasis in the XN0422/SGC7901-mock and XN0422/SGC7901-shFPR2 groups that were with the HP(2-20)/ANXA1 were significantly increased than that of the XN0422/SGC7901-mock and XN0422/SGC7901-shFPR2 group that treated with DMSO(P<0.05). Moreover, the ability of invasion and metastasis of XN0422/SGC7901-mock group was significantly increased than that of XN0422/SGC7901-shFPR2 group(P<0.05). Conclusion: FPR2 may mediate HP(2-20) and ANXA1-promoted invasion and metastasis of GC cell. |
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