| 王艺萍,张丽艳,王意忠,崔晓兰,郭姗姗.黄连解毒汤对2型糖尿病肾病大鼠肾脏的保护作用及其机制探讨[J].,2018,(5):858-862 |
| 黄连解毒汤对2型糖尿病肾病大鼠肾脏的保护作用及其机制探讨 |
| Protective Effect and Mechanism of Huanglian Jiedu Tang on Diabetic Nephropathy Rats |
| 投稿时间：2017-03-28 修订日期：2017-05-18 |
| DOI：10.13241/j.cnki.pmb.2018.05.012 |
| 中文关键词: 黄连解毒汤 糖尿病肾病 氧化应激 |
| 英文关键词: Huanglian Jiedu Tang Diabetic nephropathy Oxidative stress |
| 基金项目:院级课题基金项目（2015B-0A16） |
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| 中文摘要: |
| 摘要 目的：探讨黄连解毒汤(HLJDT)对链脲佐菌素(STZ)诱导的2型糖尿病肾病大鼠肾脏的保护作用及其可能的机制。方法：将雄性Wistar大鼠随机分为对照组(n=10)和用STZ诱导的模型组。将成功造模的大鼠随机分为模型组(n=12)、低、中和高剂量组(每组n=12)，分别给予对照组和模型组灌胃饮用水，而给其他三组分别灌胃HLJDT (60、120和240 g?kg-1?d-1)。12周后处死大鼠并测量生化指标，实时荧光定量PCR和western blot检测TGF-β1, p38MAPK 和Caspase-3的基因和蛋白表达。结果：与模型组比较，HLJDT中高剂量组体重、肾脏重、肾重体重比和FBG显著提高。与对照组比较，模型组的TG、BUN、Scr和UP 24 h均显著提高；而与模型组比较，HLJDT高剂量组均显著降低TG、BUN、Scr、UP 24 h和MDA的水平，升高NO, SOD的水平。另外，TGF-?茁1, P38MAPK 和Caspase-3的表达，模型组显著高于对照组，但经过HLJDT的治疗后，与模型组比较有显著降低。(所有取值为P<0.05，P<0.01)。结论：我们的研究提供了HLJDT是通过调控血糖、降低氧化应激的水平和下调TGF-β1, p38MAPK 和Caspase-3的表达来保护DN大鼠的实验依据。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the protective effect and possible mechanisms of Huanglian Jiedu Tang (HLJDT) on Strepto- zocin (STZ)-induced diabetic nephropathy (DN) rats. Methods: Male Wistar rats were randomly divided into the control group (n=10) and the model group received intraperitoneal injection of STZ (55 mg/kg). All successful model rats with blood glucose ≥16.7 mmol/L and urine glucose ≥4+ were then divided into the model group (n=12), the low dose one, the middle dose one and the High dose one (each n=12). Water was orally given to the rats in the control group and the model group, and HLJDT (60, 120 and 240 g?kg-1?d-1) were orally given into the other three groups respectively for 12 weeks. 12 weeks later, all the rats were sacrificed and the biochemical indica- tors such as the fasting blood glucose (FBG), triglyceride (TG), blood urea nitrogen (BUN), serum creatinine (Scr), urine protein 24 h (UP 24 h), nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. TGF-β1, p38MAPK and Cas- pase-3 gene and protein expression in kidney were detected by RT-qPCR and western blot. Results: Compared with model group, the body weight (BW) increased, the kidney weight (KW), KW/BW ratio and FBG were significantly decreased in HLJDT-Middle and HLJDT-High groups. BG, BUN, Scr and UP 24 h were significantly increased in model group compared with the control group, but de- creased markly in HLJDT-High group compared with model group. And HLJDT-Middle and HLJDT-High groups also decreased bio- chemical parameters: MDA, and increased NO and SOD compared with the model group. Moreover, the mRNA and protein expression of TGF-β1, p38MAPK and Caspase-3 in model group were significantly higher than the control group and were significantly lower than the model group after HLJDT treatment (all P<0.05, P<0.01). Conclusion: Our research provides experimental evidence that HLJDT pro- tects kidneys in DN through blood glucose control, reducing oxidative stress and down-regulating expressions of TGF-β1, p38MAPK and Caspase-3. |
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