| 冯 晨,童 梅,林福玉,徐 晨,刘金毅,姚文兵.干扰素α1b对黑色素瘤细胞A375增殖、迁移和凋亡的影响[J].,2018,(3):453-457 |
| 干扰素α1b对黑色素瘤细胞A375增殖、迁移和凋亡的影响 |
| Effects of IFN-α1b on the Proliferation, Migration and Apoptosis of Melanoma Cell A375 |
| 投稿时间：2017-04-30 修订日期：2017-05-24 |
| DOI：10.13241/j.cnki.pmb.2018.03.011 |
| 中文关键词: 干扰素α1b 黑色素瘤 增殖 迁移 凋亡 |
| 英文关键词: IFN-α1b Melanoma Proliferation Migration Apoptosis |
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| 中文摘要: |
| 摘要 目的：研究干扰素α1b(IFN-α1b)对人恶性黑色素瘤细胞A375增殖、迁移和凋亡的影响。方法：采用体外培养的黑色素瘤细胞A375，通过MTT法检测和比较IFN-α1b和IFN-α2b对A375细胞的增殖的抑制作用；细胞划痕实验分析IFN-α1b对A375细胞迁移的影响；Annexin V-FITC/PI染色后采用荧光显微镜观察和流式细胞术分析IFN-α1b对A375细胞凋亡的影响。结果：MTT结果显示随着IFN-α1b和IFN-α2b浓度的增加，A375细胞的生长抑制率逐渐升高，药物作用48 h和72 h后，IFN-α1b作用的A375细胞生长抑制率明显高于IFN-α2b，IFN-α1b和IFN-α2b作用48 h的IC50分别为(22.69±1.52)μg/mL 和(35.69±1.01)μg/mL(P<0.01)；IFN-α1b和IFN-α2b作用72 h的IC50分别为(10.00±0.98)μg/mL和(25.02 ±0.44)μg/mL(P<0.01)。细胞划痕实验显示IFN-α1b能够使A375细胞的迁移指数和迁移能力降低，且随着IFN-α1b药物浓度的增加抑制细胞迁移作用增强。随着50 μg/mL IFN-α1b作用的A375细胞的凋亡率为(29.31±0.45)%，较对照组(8.21±1.36)%相比明显上升(P<0.01)。结论：IFN-α1b对人恶性黑色素瘤细胞A375具有生长抑制作用，与IFN-α2b相比，IFN-α1b的抑制作用更加显著；IFN-α1b能够降低细胞A375的迁移能力并诱导其凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the influence of IFN-α1b on the proliferation, migration and apoptosis of melanoma cell A375. Methods: Melanoma cell A375 was cultured in vitro. MTT assays were used to determine and compare the anti-proliferation ef- fects of IFN-α1b and IFN-α2b on each cell group. The scratch wound assay was used to determine the influence of IFN-α1b on the mi- gratory activity of melanoma cell A375. By using Annexis V-FITC/PI staining, fluorescent microscopy and flow cytometric analysis were used to analyze the apoptosis of melanoma cell A375. Results: MTT results showed that IFN-α1b and IFN-α2b were both able to inhibit the proliferation of melanoma cell A375 in a dose- and time-dependent manner. With the increase of concentrations of IFN-α1b and IFN-α2b, the anti-proliferation effects on melanoma cell A375 increased gradually. After 48 h and 72 h, melanoma cell A375 was more susceptible to IFN-α1b than to IFN-α2b. After 48 h, IC50 of IFN-α1b and IFN-α2b were (22.69±1.52) μg/mL and (35.69 ±1.01) μg/mL(P<0.01); After 72 h, IC50 of IFN-α1b and IFN-α2b were (10.00±0.98) μg/mL and (25.02 ±0.44) μg/mL(P<0.01). The scratch wound assay results indicated that IFN-α1b treatment groups led to a decrease in migration index and migration ability of melanoma cell A375, and with the increase of the concentration of IFN-α1b, the inhibition effects of migration on melanoma cell A375 increased. The apoptosis rate of melanoma cell A375 treated with 50 μg/mL IFN-α1b was (29.31±0.45)%, which was significantly higher than that of the control group of (8.21±1.36)%(P <0.01). Conclusion: IFN-α1b was able to inhibit melanoma cell A375 growth in vitro, and the anti- proliferation effects of IFN-α1b were more significant than that of IFN-α2b; IFN-α1b was also able to inhibit the migration ability and to induce apoptosis of melanoma cell A375. |
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