| 冯 月,陈珍珍,胡 海,于 慧,王占黎.microRNA-151-5p在肾血管性高血压大鼠血管内皮细胞中的表达及意义[J].,2018,(2):239-242 |
| microRNA-151-5p在肾血管性高血压大鼠血管内皮细胞中的表达及意义 |
| Expression and Significance of MicroRNA-151-5p in the Vascular Endothelial Cells of Renovascular Hypertensive Rats |
| 投稿时间：2017-08-04 修订日期：2017-08-26 |
| DOI：10.13241/j.cnki.pmb.2018.02.010 |
| 中文关键词: 肾血管性高血压 miR-151-5p 靶基因 |
| 英文关键词: Renovascular hypertension MiR-151-5p Target gene |
| 基金项目:国家自然科学基金项目（81460049；81560057） |
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| 中文摘要: |
| 摘要 目的：研究microRNA-151-5p(miR-151-5p)在两肾一夹(2K1C)肾血管性高血压大鼠中的表达，并为miR-151-5p参与调节血管内皮细胞功能提供理论依据。方法：建立2K1C大鼠模型，获得胸主动脉血管内皮，采用荧光定量PCR技术检测血管内皮细胞中miR-151-5p的表达，通过数据库及生物信息学软件预测miR-151-5p的靶基因，并对靶基因进行GO富集和KEGG pathway分析。结果：与假手术组大鼠相比较，实验组大鼠胸主动脉血管内皮细胞miR-151-5p的表达量显著升高(P<0.05)。GO分析显示miR-151-5p的靶基因参与蛋白加工水解、Notch受体加工等多种生物学功能(P<0.01)；KEGG pathway分析显示miR-151-5p的靶基因参与Notch信号通路、血管平滑肌收缩、代谢途径、细菌感染和RNA转运等信号通路。结论：2K1C大鼠血管内皮细胞中miR-151-5p表达升高，可能通过对其靶基因APH1A的调控参与血管内皮细胞功能调节。 |
| 英文摘要: |
| ABSTRACT Objective: To study the expression of microRNA-151-5p (miR-151-5p) in two kidney one clip (2K1C) renovascular hypertensive rats, and provide a theoretical guidance for miR-151-5p to participate in the regulation of vascular endothelial cell function. Methods: 2K1C rat model was established to obtain the endothelium of thoracic aorta. The expression of miR-151-5p in vascular endothelial cells was detected by fluorescent quantitative PCR. We also predicted target genes of miR-151-5p by database and bioinformatics software. Furthermore, we analyzed the function of target gene by KEGG and GO pathways. Results: Compared with the sham group, the expression of miR-151-5p in thoracic aorta endothelial cells in experimental group was significantly higher (P<0.05). In GO analysis, the target genes of miR-151-5p were enriched in many biological process,such as regulation of protein processing and regulation of Notch receptor processing(P<0.01). In KEGG pathway, the target genes of miR-151-5p were involved in Notch signaling pathway, vascular smooth muscle contraction, metabolic pathway, bacterial infection and RNA transport pathway. Conclusion: MiR-151-5p expression increased in 2K1C rats, it may regulate the function of vascular endothelial cell by its target gene APH1A. |
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