| 凯赛尔江·多来提,古丽美热·艾买如拉,吐尔逊江·达地汗,迪力夏提·艾尼,廖礼彬,迪力木拉提·艾尼,李 杰,陈胜国,古丽尼沙·克力木.人类胚胎三叉神经运动核的定位研究[J].,2018,(2):226-229 |
| 人类胚胎三叉神经运动核的定位研究 |
| A Study on the Location of Tigeminal Motor Nucleas in the Human Fetus |
| 投稿时间：2017-07-22 修订日期：2017-08-17 |
| DOI：10.13241/j.cnki.pmb.2018.02.007 |
| 中文关键词: 三叉神经运动核 DiI 胚胎 |
| 英文关键词: Trigeminal motor nucleus DiI Human fetus |
| 基金项目:国家自然科学基金项目(31360284) |
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| 中文摘要: |
| 摘要 目的：探讨人类胚胎三叉神经运动核的位置及细胞分布特征。方法：选用非疾病死亡的引产胚胎标本4例，胎龄20-26周(根据胎儿B超检测和孕妇末次月经日期来计算并获得胎龄)，在获取标本1-4小时内，对标本进行灌流固定。其中，2例标本在手术显微镜下开颅取出脑干，石蜡包埋、冰冻切片、HE染色、光学显微镜下观察、照相，其余2例标本在手术显微镜下开颅、分离三叉神经根，注入DiI结晶体、在37 ℃恒温箱内保存3个月，取出脑干、明胶包埋、冰冻切片，在荧光显微镜下观察、照相。结果：①胚胎三叉神经运动核位于脑桥三叉神经根连脑水平；②三叉神经运动核为多级细胞、胞体较大、位于三叉神经根入脑桥的一束纤维的腹内侧；③三叉神经运动核呈椭圆形，神经核细胞呈大小不等,体积较大,胞体呈多角形。结论：人类胚胎三叉神经运动核为大型的多级细胞成群所形成的核团，位于脑桥三叉神经根连脑水平。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the location of trigeminal motor nucleas in human fetus. Methods: 4 cases of human embryonic specimens which non-disease deaths of the fetus induced labor, gestational age 20 -26weeks(The age of gestational age was obtained by based on the date of the last menstrual period of the pregnant woman, and the ultrasound examination and the measurement of the specimen), obtaining the specimen within 1 to 4 hours. The specimens were fixed. 2 specimens, take out the brain stem under the operation microscope, and the paraffin embedding, frozen section, HE staining, and observe and take photos under optical microscope. In the rest 2 specimens, under operation microscope, separate root of the trigeminal nerve and inject with DiI, keep 3 months in incubator at 37 ℃, and take out the brain stem, gelatin embedding, frozen section, observe and take photos under the fluorescence microscope. Results:①The trigeminal motor nucleus is located in level of pons connect with the root of trigeminal nerve. ②The trigeminal motor nucleus is a multistage cell, cell body is larger and located in the ventromedial part of the pons connect with root of the trigeminal nerve. ③The trigeminal motor nucleus is oval shape, nerve cells are unequal size, the cell is larger, cell body is polygon shape. Conclusion: The trigeminal motor nucleus in human fetus is a large nucleus formed by multistage cells, and located in level of pons connect with the root of trigeminal nerve. |
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