| 王忠杰,余 平,朱秀坤,贡 雪,马长剑.microRNA-145表达对宫颈癌Hela细胞增殖及凋亡的影响[J].,2018,(1):57-60 |
| microRNA-145表达对宫颈癌Hela细胞增殖及凋亡的影响 |
| Effect of microRNA-145 Expression on Proliferation and Apoptosis of Cervical Cancer Hela Cells |
| 投稿时间：2017-09-29 修订日期：2017-10-22 |
| DOI：10.13241/j.cnki.pmb.2018.01.012 |
| 中文关键词: 宫颈癌 微小核糖核酸145 Hela细胞 增殖 凋亡 |
| 英文关键词: Cervical carcinoma Micro Ribonucleic Acid 145 Hela cells Proliferation Apoptosis |
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| 中文摘要: |
| 摘要 目的：探讨微小核糖核酸145（microRNA-145）表达对宫颈癌Hela细胞增殖及凋亡的影响。方法：实验室常规培养宫颈癌Hela细胞并分为4组，空白（Blank）组（Hela细胞+RPMI1640）、microRNA-145组（Hela细胞+RPMI1640+microRNA-145-5p mimics）、阴性序列（NC）组（Hela细胞+RPMI1640+NC）、Mock组（Hela细胞+RPMI1640+Lipofectamine 2000），记录各组Hela细胞转染率，采用实时荧光定量聚合酶链锁反应（QRT-PCR）检测各组Hela细胞中microRNA-145的表达水平，采用四甲基偶氮唑蓝（MTT）比色法检测Hela细胞增殖情况，采用4',6-二脒基-2-苯基吲哚（DAPI）染色法判断Hela细胞凋亡情况。结果：本研究中，各组Hela细胞转染率均＞80%；microRNA-145组microRNA-145的表达显著高于Blank组、NC组和 Mock组，差异有统计学意义（P＜0.05）。转染24 h、48 h、72 h后，microRNA-145组490 nm波长处的光密度值（OD490值）较转染0h后明显降低，转染48 h、72 h后，Blank组、NC组、Mock组OD490值较转染0 h后时明显升高，转染24 h、48 h、72 h后，microRNA-145组OD490值均低于Blank组、NC组、Mock组，差异有统计学意义（P＜0.05）。DAPI染色后，microRNA-145组Hela细胞凋亡率高于Blank组、NC组、Mock组，差异有统计学意义（P＜0.05）。转染后，Blank组、NC组、Mock组的microRNA-145表达率、OD490值、DAPI染色后Hela细胞凋亡率比较差异均无统计学意义（P＞0.05）。结论：microRNA-145表达上调可抑制宫颈癌Hela细胞增殖，并促进Hela细胞凋亡，通过药物调控microRNA-145表达有望成为宫颈癌治疗的新靶点。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of micro Ribonucleic Acid 145 (microRNA-145) expression on the proliferation and apoptosis of cervical cancer Hela cells. Methods: The cervical cancer Hela cells were cultured routinely in the laboratory and divided into blank group (Hela cell + RPMI1640), microRNA-145 group (Hela cell + RPMI1640 + microRNA-145-5p mimics), NC group (Hela cell + RPMI1640 + NC) and Mock group (Hela cell + RPMI1640 + Lipofectamine 2000). The transfection rate of Hela cells in each group was recorded, the expression of microRNA-145 in Hela cells was detected by real-time quantitative polymerase chain reaction (QRT-PCR), the proliferation of Hela cells was detected by four methyl blue tetrazolium (MTT) colorimetric assay, the apoptosis of Hela cells was determined by 4',6-diamidino-2-phenylindole (DAPI) staining. Results: In this study,the transfection rate of Hela cells in each group was more than 80%; the expression of microRNA-145 in microRNA-145 group was significantly higher than that in blank group,NC group and Mock group, the differences were statistically significant (P<0.05). After transfection of 24 h, 48 h and 72 h, the light intensity values(OD490) value of 490nm wave in microRNA-145 group were significantly lower than that of transfection of 0 h; after transfection of 48 h and 72 h, the OD490 value of blank group, NC group and Mock group were significantly higher than that of transfection of 0 h; after transfection of 24 h, 48 h and 72 h, the OD490 values in the microRNA-145 group were lower than those in the blank group, the NC group and the Mock group, the differences were statistically significant (P<0.05). After DAPI staining, the apoptosis rate of Hela cells in microRNA-145 group was higher than that in blank group, NC group and Mock group, the difference was statistically significant (P<0.05). After transfection, there were no significant differences in the microRNA-145 expression rate, OD490 value and Hela cell apoptosis rate after DAPI staining in blank group, NC group and Mock group(P>0.05). Conclusion: Up-regulation of microRNA-145 ex- pression can inhibit the proliferation of cervical cancer Hela cells and promote apoptosis of Hela cells,and the regulation of microR- NA-145 expression by drugs is expected to be a new target for cervical cancer treatment. |
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