| 李 超,袁 鹏,颜昭勇,李嘉琦,杨 懿,谢欣恬,张洪新,牟 佼.CRY2分子对肝癌细胞生长和转移的影响[J].,2017,17(36):7030-7035 |
| CRY2分子对肝癌细胞生长和转移的影响 |
| Effects of CRY2 on the Growth and Metastasis of Hepatocellular Carcinoma Cells |
| 投稿时间：2017-08-19 修订日期：2017-09-10 |
| DOI：10.13241/j.cnki.pmb.2017.36.007 |
| 中文关键词: CRY2 生物节律 生长 转移 肝细胞肝癌 |
| 英文关键词: CRY2 Circadian rhythm Growth Metastasis HCC |
| 基金项目:国家自然科学基金项目（81572304，81600478） |
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| 中文摘要: |
| 摘要 目的：探讨节律分子CRY2（cryptochrome circadian clock 2）在肝细胞肝癌（HCC）中的表达和对肝癌细胞生长、转移的影响。方法：利用免疫组织化学染色法，分析65例HCC组织中CRY2分子的表达；利用233例HCC公共数据验证CRY2分子在HCC组织中的表达变化；实时定量PCR和Western blot检测肝癌细胞中CRY2的表达。MTS法和Transwell法，分别检测CRY2对肝癌细胞生长和转移的影响。结果：对照组CRY2阳性率为87.7%（57/65），而在肝癌组织中49.2%（32/65）CRY2阳性，CRY2在HCC组织中的表达显著低于对照组，差异有统计学意义（t=10.61，P<0.0001）。233例HCC公共数据（GSE14520）中，癌组织CRY2表达显著下调，差异具有统计学意义（6.663 vs 6.160，P<0.0001）。4株肝癌细胞株中CRY2表达与正常肝细胞株相比，CRY2表达同样显著下调。在肝癌SMMC-7721和Hep 3B细胞中，与对照组比，过表达CRY2组细胞生长能力显著降低，差异有统计学意义（SMMC-7721：0.899 vs 0.473，P<0.0001；Hep 3B：0.785 vs 0.435，P<0.0001）。在肝癌SMMC-7721和Hep 3B细胞中，过表达CRY2组细胞侵袭能力显著降低（SMMC-7721：侵袭细胞数216.33 vs 62.33，P=0.001；Hep 3B：侵袭细胞数169.67 vs 52.33，P=0.015）；结论：CRY2分子在HCC中表达下调，同时高表达CRY2会抑制肝癌细胞生长和转移。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the expression of CRY2 in hepatocellular carcinoma (HCC) and its effects on the growth and metastasis of HCC cells. Methods: The expression of CRY2 in 65 HCC patients was analyzed by immunohistochemical staining (IHC). Using public dataset from 233 HCC patients to analyze and validate the expression of CRY2. Real-time quantitative PCR and Western blot were used to detect the expression of CRY2 in HCC cells. The effect of CRY2 on the growth and metastasis of HCC cells were detected by MTS and Transwell. Results: The positive rate of CRY2 expression in the control group was 87.7% (57/65), and was 49.2% (32/65) in HCC tissues. The expression of CRY2 protein in HCC tissues was significantly lower than that in peritumor tissues, the difference was statistically significant (t=10.61 P<0.0001). The expression of CRY2 in 233 cases HCC tissues (GSE14520) was significantly lower than that in adjacent tissues, the difference was statistically significant (6.663 vs 6.160, P<0.0001). The expression of CRY2 in four HCC cell lines was also significantly down-regulated compared with normal hepatocyte cell lines. Overexpression of CRY2 group compared with the control group, cell growth was significantly reduced, the difference between the two groups was statistically significant (SMMC-7721: 0.899 vs 0.473, P<0.0001; Hep 3B: 0.785 vs 0.435，P<0.0001). The ability of cell invasion was significantly decreased in overexpression CRY2 group than that in the control group (SMMC-7721: invasion cell number 216.33 vs 62.33, P=0.001; Hep 3B: invasion cell number 169.67 vs 52.33，P=0.015). Conclusion: The expression of CRY2 is low in HCC, and CRY2 can inhibit growth and metastasis of HCC cells. |
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