| 黄丽丽,吴玉璘,许豪勤,姜 标,王溢慧,郭海建.前S1抗原检测在献血人群的HBV感染筛查中的应用[J].,2017,17(34):6745-6748 |
| 前S1抗原检测在献血人群的HBV感染筛查中的应用 |
| Application of Detection of Pre-S1 Antigen in Screening Blood Donators with HBV Infection |
| 投稿时间：2016-12-31 修订日期：2017-01-28 |
| DOI：10.13241/j.cnki.pmb.2017.34.031 |
| 中文关键词: 前S1抗原 谷丙转氨酶 乙肝病毒表面抗原 乙肝病毒DNA |
| 英文关键词: Pre-S1 antigen ALT HBsAg HBV-DNA |
| 基金项目:江苏省科学技术厅省属公益院所能力提升项目（BM2015020） |
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| 中文摘要: |
| 摘要 目的：通过比较献血人群中谷丙转氨酶、乙肝表面抗原和前S1抗原检测与HBV-DNA检测结果的一致性，探讨前S1抗原检测在献血人群中筛查乙肝病毒感染者的应用价值。方法：1080份献血样本中，采用ELISA法检测前S1抗原，PCR法检测HBV-DNA，分析前S1抗原检测与HBV-DNA检测的一致性。结果：①1000例合格的献血人群中，前S1抗原的阳性率为0.3%，HBV-DNA阳性率为0.1%，两者呈中度一致性(Kappa值=0.499)，且有统计学意义(P<0.05)。②1080例样本中，ALT、HBsAg、前S1抗原的阳性率分别为5.83%、1.76%、1.67%，HBV-DNA阳性率为0.93%，ALT检测法与HBV-DNA检测无一致性(Kappa值=-0.0162)；HBsAg检测与HBV-DNA检测呈高度一致性(Kappa值=0.6160)，前S1抗原检测与HBV-DNA检测呈高度一致性(Kappa值=0.7108)，二者均有统计学意义(P<0.05)。③ALT和HBsAg两项联合检测及ALT、HBsAg和前S1抗原三项联合检测的阳性率分别为7.41%、7.69%，HBV-DNA阳性率为0.93%。两项联合检测法与HBV-DNA检验结果一致(Kappa值=0.1866)，三项联合检测法与HBV-DNA检验结果一致(Kappa值=0.2019)，均有统计学意义(P <0.05)。结论：前S1抗原检测与HBV-DNA检测有较高一致性，能够减少献血人群中HBV感染的漏检；将ALT、HBsAg和前S1抗原进行联合检测能够提高HBV感染筛查率。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the value of determining Pre-S1 antigen in screening blood donators with HBV infection by comparing the consistency between ALT, HBsAg, Pre-S1 antigen tests and HBV-DNA tests for blood donators. Methods: A total of 1080 samples were respectively tested by Pre-S1 antigen with an ELISA method and HBV-DNA with a PCR method. Based on the results of tests, the consistency between Pre-S1 antigen and HBV-DNA tests was analyzed. Results: ①In 1000 cases of qualified samples, the positive rate of Pre-S1 antigen was 0.3%, and the positive rate of HBV-DNA was 0.1%, these two test indexes had a medium-degree consistency (Kappa value 0.499)(P<0.05). ②In 1080 cases of blood samples, the positive rates of ALT, HBsAg and Pre-S1 antigen were 5.83%，1.76% and 1.67% , and the positive rate of HBV-DNA was 0.93%. There was no statistical difference in the positive rate of HBsAg and Pre-S1 antigen but ALT respectively compared that of HBV-DNA (P>0.05). There were statistical difference for the positive rate of ALT, HBsAg and Pre-S1 antigen respectively compared that of HBV-DNA (P< 0.05). Kappa's test showed no consistency between ALT and HBV-DNA (Kappa value -0.0162), high-degree consistency between HBsAg and HBV-DNA (Kappa value 0.6160) and high-degree consistency between Pre-S1 antigen and HBV-DNA (Kappa value 0.7108) were found(P<0.05). ③In 1080 cases of blood samples, the positive rate of combined ALT and HBsAg was 7.41%, the positive rate of combined ALT plus HBsAg plus Pre-S1 antigen was 7.69%, and the positive rate of HBV-DNA was 0.93%. There were statistical difference in the positive rates of the combined with Pre-S1 antigen and without Pre-S1 antigen respectively compared that of HBV-DNA (P<0.05). Kappa's test showed consistency between combined ALT plus HBsAg and HBV-DNA with a Kappa value of 0.1866, as well as a consistency between combined ALT plus HBsAg plus Pre-S1 antigen and HBV-DNA with a Kappa value of 0.2019 (P<0.05). Conclusion: The test of Pre-S1 antigen had a superior consistency with test of HBV-DNA, and could be used to screen the untested donated blood samples with HBV. Furthermore, a combined test of Pre-S1 antigen, ALT and HBsAg could increase the screening rates of donated blood samples with HBV. |
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