| 赵 超,丁 兰,张 丹,王丽亮,韩 辉.血浆游离DNA的临床应用新进展[J].,2017,17(33):6580-6583 |
| 血浆游离DNA的临床应用新进展 |
| New Clinical Researches on Plasma Cell-free DNA |
| 投稿时间：2016-12-28 修订日期：2017-01-23 |
| DOI：10.13241/j.cnki.pmb.2017.33.041 |
| 中文关键词: 血浆游离DNA 肿瘤 DNA甲基化 产前诊断 心肌梗死 |
| 英文关键词: Plasma free DNA Tumor DNA methylation Prenatal diagnosis Myocardial infarction |
| 基金项目:黑龙江省自然科学基金项目(C201245) |
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| 中文摘要: |
| 摘要：血浆游离DNA（Plasma cell-free DNA, cf-DNA）游离于细胞外的机体内源性DNA, cf-DNA广泛存在于血液、脑脊液、滑膜液、支气管肺泡灌洗液和羊水等体液中。多数血浆DNA与蛋白质结合成为复合物，仅有少部分为游离的片段，受实验方法灵敏性和特异性的限制，cf-DNA的研究进展缓慢。随着分子检测技术的发展，虽然在健康人群的血浆中也可测量到一定量的cf-DNA，但是cf-DNA被发现在肿瘤、自身免疫性疾病、创伤和炎性反应等病理过程中有改变。目前cf-DNA在肿瘤的诊断和预后分析、产前诊断、感染性疾病等中均有应用价值。 |
| 英文摘要: |
| ABSTRACT: Plasma cell-free DNA(cf-DNA)is a cell-free endogenous status of extracellular DNA. Now believed cf-DNA is widely present in blood, cerebrospinal fluid, synovial fluid, bronchoalveolar lavage fluid and amniotic fluid and other body fluids. Most plasma protein binding with DNA to become complex but a few of the free fragments. The progress of cf-DNA is restricted to the sensitivity and specificity of experimental methods. With the development of molecular detection techniques, although a certain amount cf-DNA in the plasma of healthy people could be detecte, dut cf-DNA was found to have changed in the pathological process of cancer, autoimmune sieases, and inflammatory reactions in trauma. Currently cf-DNA is value in the diagnosis and prognosis of tumors analyzed, prenatal di- agnosis, and other infectious diseases. |
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