| 李 辉,高胜利,柳 洋,孙向荣,郭菲菲.Nesfatin-1对大鼠摄食、胃酸分泌、胃运动及胃排空的影响[J].,2017,17(32):6237-6241 |
| Nesfatin-1对大鼠摄食、胃酸分泌、胃运动及胃排空的影响 |
| The Effects of Nesfatin-1 on Food Intake, Gastric Acid Secretion, Gastric Motility and Gastric Emptying in Rats |
| 投稿时间：2017-05-11 修订日期：2017-06-07 |
| DOI：10.13241/j.cnki.pmb.2017.32.007 |
| 中文关键词: Nesfatin-1 摄食 胃酸分泌 胃运动 胃排空 |
| 英文关键词: Nesfatin-1 Food intake Stomach acid secretion Stomach movement Gastric emptying |
| 基金项目:山东省优秀中青年科学基金项目（BS2014YY009） |
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| 中文摘要: |
| 摘要 目的：观察Nesfatin-1对大鼠摄食、胃酸分泌、胃运动及胃排空的影响并探究其可能机制。方法：将大鼠随机分为摄食实验组、胃酸实验组、胃运动实验组以及胃排空实验组。大鼠经腹内侧核置管后给予nasfatin-1，检测大鼠摄食量，使用NaOH滴定法测定大鼠胃酸分泌，记录清醒大鼠胃运动，以比色法测定大鼠胃排空。结果：低剂量和高剂量nesfatin-1均减少2小时累积食物摄入量；高剂量组4小时累积食物摄入量仍显著低于NS对照组。Nesfatin-1能够抑制2-DG对胃酸分泌的促进作用。SHU9119能够部分阻断nesfatin-1对2-DG的抑制作用。Nesfatin-1能够抑制胃运动及胃排空，SHU9119可部分阻断nesfatin-1对胃运动及胃排空的抑制作用。结论：Nesfatin-1能够调控大鼠摄食、胃酸分泌、胃运动及胃排空，黑皮质素信号通路可能也参与该调控过程。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effects of Nesfatin-1 on the food intake, gastric acid secretion, gastric motility and gastric emptying in rats and explore the underlying mechanisms. Methods: Rats were randomly divided into four groups: feeding group, gastric acid experimental group, gastric motility group and gastric emptying group. Rats were administered nasfatin-1 via the ventral medial nucleus, the changes of food intake were detected, the change of gastric acid secretion was detected by NaOH titration method, the stomach movement was recorded and the change of gastrc emptying was determined by colorimetric method. Results: Low and high dose of nesfatin-1 reduced the food intake; the 4 hours' cumulative food intake of high dose group was still significantly lower than that of the NS control group(P<0.05). Nesfatin-1 inhibited the promoting effect of 2-DG on the gastric acid secretion. SHU9119 partially blocked the inhibitory effect of nesfatin-1 on 2-DG. Nesfatin-1 inhibited the gastric motility and gastric emptying. SHU9119 partially blocked the effect of nesfatin-1 on the gastric motility and gastric emptying inhibition. Conclusion: Nesfatin-1 regulated the feeding, gastric acid secretion, gastric motility and gastric emptying, melanocortin signaling pathways might also be involved in this regulatory process. |
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