| 张秀玲,杨晓静,范引侠,李芬霞,贾小文.ox-AAT、HMGB1、MMP-9在胎膜早破孕妇中的表达及意义[J].,2017,17(30):5929-5932 |
| ox-AAT、HMGB1、MMP-9在胎膜早破孕妇中的表达及意义 |
| Expressions and Significances of ox-AAT, HMGB1 and MMP-9 in the Pregnant Women with Premature Rupture of Membranes |
| 投稿时间：2017-05-07 修订日期：2017-05-29 |
| DOI：10.13241/j.cnki.pmb.2017.30.030 |
| 中文关键词: 氧化型α1-抗胰蛋白酶 高迁移率族蛋白B1 基质金属蛋白酶-9 胎膜早破 |
| 英文关键词: Oxidationα1-antitrypsin High mobility group box 1 Matrix metalloproteinase-9 Premature rupture of membrane |
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| 中文摘要: |
| 摘要 目的：探讨氧化型α1-抗胰蛋白酶(ox-AAT)、高迁移率族蛋白B1(HMGB1)、基质金属蛋白酶-9(MMP-9)在胎膜早破(PROM)孕妇中的表达及意义，为胎膜早破孕妇的早期诊断和治疗提供参考依据。方法：收集我院2016年1月到2017年1月收治的76例足月胎膜早破(TPROM)、44例未足月胎膜早破(PPROM)及50例正常足月孕妇作为研究对象，采用ELISA法检测母血及新生儿脐血ox-AAT、HMGB1、MMP-9水平，采用免疫组化法检测胎膜组织中ox-AAT、HMGB1、MMP-9蛋白的表达，比较三组母血、新生儿脐血及胎膜组织中ox-AAT、HMGB1、MMP-9水平，分析胎膜组织中ox-AAT、HMGB1、MMP-9之间相关性。结果：TPROM组与PPROM组母血、新生儿脐血、胎膜组织中ox-AAT、HMGB1、MMP-9水平均显著高于对照组(P＜0.05)，且PPROM组以上指标均显著高于TPROM组(P＜0.05)。胎膜组织中MMP-9的表达与ox-AAT呈正相关(r＝0.779，P＜0.05)，与HMGB1的表达呈正相关(r＝0.811，P＜0.05)。结论：母血、新生儿脐血、胎膜组织中ox-AAT、HMGB1、MMP-9变化与PROM及早产的发生相关，胎膜组织中MMP-9、ox-AAT、HMGB1均可能作为临床胎膜早破诊断以及治疗效果评价的参考指标。抗胰蛋白酶 |
| 英文摘要: |
| ABSTRACT Objective: To explore the expressions and significances of α1-antitrypsin（ox-AAT）, high mobility group box-1 protein（HMGB1） and matrix metalloprotein-9（MMP-9） in pregnant women with premature rupture of membranes and provide references for the early diagnosis and treatment of premature rupture of membranes. Methods: 76 cases of pregnant women with premature rupture (TPROM) 44 cases of preterm premature rupture of membranes (PPROM) and 50 cases of normal pregnant women in our hospital from January 2016 to January 2017 were selected as the research object, ELISA method was used to detect the maternal and neonatal umbilical cord blood ox-AAT, HMGB1 and MMP-9 levels, ox-AAT, HMGB1, MMP-9 protein expressions in the fetal membranes were detected by by immunohistochemistry, the ox-AAT, HMGB1, MMP-9 expressions in the maternal blood and neonatal cord blood and fetal tissue were compared between three groups. Results: The ox-AAT HMGB1, maternal blood MMP-9 in the maternal blood and neonatal cord blood and fetal tissue of TPROM group and PPROM group,were significantly higher than those of the control group (P < 0.05), which were significantly higher in the PPROM group than those of the TPROM group(P<0.05). The expression of MMP-9 in fetal membranes was positively correlated with ox-AAT (r=0.779, P<0.05 and HMGB1 expressions (r=0.811, P<0.05). Conclusion: The ox-AAT, HMGB1 and MMP-9 expressions were correlated with the incidence of PROM and preterm birth. The ox-AAT, HMGB1, MMP-9 expressions in the fetal tissue could be used as reference index for the diagnosis and the evaluation of therapeutic effect of clinical premature rupture of membranes. |
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