| 赵晓红,冯 丽,张百红,马文英,胡文梅.二甲双胍联合卡铂对三阴性乳腺癌细胞增殖的影响[J].,2017,17(30):5837-5839 |
| 二甲双胍联合卡铂对三阴性乳腺癌细胞增殖的影响 |
| Effects of Metformin combined with Carboplatin on the Proliferation of Triple Negative Breast Cancer Cells |
| 投稿时间：2017-04-27 修订日期：2017-05-20 |
| DOI：10.13241/j.cnki.pmb.2017.30.008 |
| 中文关键词: 二甲双胍 卡铂 三阴性乳腺癌细胞 细胞增殖 |
| 英文关键词: Metformin Carboplatin Triple negative breast cancer cell Proliferation |
| 基金项目:甘肃省自然科学基金项目（1308RJZA181） |
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| 中文摘要: |
| 摘要 目的：探讨二甲双胍联合卡铂对三阴性乳腺癌细胞MDA-MB-231增殖的影响。方法：体外培养三阴性乳腺癌细胞MDA-MB-231，分别给予不同浓度(0、2.5、5、10 mmol/L) 二甲双胍和20 μmol/L卡铂处理后，采用MTT实验、平板克隆实验检测二甲双胍联合卡铂对MDA-MB-231细胞增殖的影响。结果：MTT实验结果显示：二甲双胍抑制MDA-MB-231细胞的增殖，5、10 mM组细胞OD490值均显著低于对照组(P <0.05)，且10 mM组细胞OD490值明显低于其他各组(P<0.05)；与单药相比，二甲双胍和卡铂联用组细胞生长抑制率显著升高，差异具有统计学意义(P<0.05)。平板克隆实验结果显示：与单药相比，二甲双胍和卡铂联用组细胞的克隆形成抑制率率显著升高，差异具有统计学意义(P<0.05)。结论：二甲双胍联合卡铂能够有效的抑制三阴性乳腺癌细胞MDA-MB-231的增殖。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of metformin combined with carboplatin on the proliferation of triple negative breast cancer cells MDA-MB-231. Methods: MDA-MB-231 cell was cultured in vitro and treated with different concentrations (0, 2.5, 5, 10 mmol/L) of metformin and 20 μmol/L carboplatin, respectively. MTT and clone formation assays were used to detect the effect of metformin combined with carboplatin on the proliferation of MDA-MB-231 cells. Results: MTT assay showed that metformin inhibited the proliferation of MDA-MB-231 cells. The cells OD490 values in 5 or 10mM group were significantly lower than that of the control group (P <0.05), and the OD490 values in 10 mM group were significantly lower than those in the other groups (P <0.05). Compared with monotherapy, the cell growth inhibition rate of metformin combined with carboplatin was significantly increased(P<0.05). The results of plate clone formation assay showed that compared with monotherapy, the cell clone formation inhibition rate of metformin combined with carboplatin was significantly increased(P<0.05). Conclusion: Metformin combined with carboplatin could effectively reduce the proliferation of triple negative breast cancer cell line MDA-MB-231. |
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