| 高 鹏,张克英,周 艳,林艳云,张俊平,陈永斌,郭 玲,刘军叶,曾丽华,李予蓉,丁桂荣,郭国祯.1840 MHz射频辐射对大鼠血脑屏障通透性的影响[J].,2017,17(30):5827-5831 |
| 1840 MHz射频辐射对大鼠血脑屏障通透性的影响 |
| Effects of 1840 MHz Radio Frequency Electromagnetic Radiation on the Permeability of Blood-brain Barrier in Rats |
| 投稿时间：2017-03-23 修订日期：2017-04-20 |
| DOI：10.13241/j.cnki.pmb.2017.30.006 |
| 中文关键词: 射频辐射 血脑屏障 通透性 大鼠 |
| 英文关键词: Radio frequency electromagnetic radiation Blood-brain barrier Permeability Rat |
| 基金项目:国家自然科学基金重点项目（51437008）；陕西省科技计划项目（2014KTCQ03-02，2016SF-018） |
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| 中文摘要: |
| 摘要 目的：探讨环境射频辐射暴露对雄性SD大鼠血脑屏障通透性的影响。方法：36只成年雄性SD大鼠随机分为暴露组和假暴露组，对暴露组大鼠进行频率为1840 MHz、比吸收率（SAR值）为2 W/kg、每日1 h、连续7 d的全身辐照。硝酸镧示踪透射电镜法和白蛋白免疫组化法观察暴露后大鼠脑皮质血脑屏障超微结构和通透性，免疫印迹法检测血脑屏障紧密连接相关蛋白ZO-1表达水平。结果：透射电镜结果显示，假暴露组镧颗粒仅出现在大鼠脑微血管管腔内；而在暴露组，除微血管管腔外，在局部脑皮质微血管内皮基底膜处和周围脑实质间亦可见镧颗粒沉积。免疫组化染色显示，假暴露组大鼠脑皮质微血管周围未见白蛋白渗出，而暴露组大鼠脑皮质微血管周围有明显白蛋白沉积。上述结果提示，射频辐射暴露可能增加血脑屏障通透性。免疫印迹结果显示，与假暴露组相比，暴露组大鼠脑皮质ZO-1蛋白表达水平明显降低（P＜0.001），差异具有统计学意义。结论：1840 MHz射频辐射可诱导血脑屏障发生一定程度地开放，紧密连接相关蛋白ZO-1表达水平的改变可能参与该过程。 |
| 英文摘要: |
| ABSTRACT Objective: With the development of modern communication and the popularization of mobile phone, the exposure level of radiofrequency (RF) radiation emitted from mobile phone has been increased unprecedentedly, which leads to extensive public concern about the influences of RF radiation on human health. This investigation was designed to explore the effects of environmental radio frequency fields on the permeability of blood brain barrier (BBB). Methods: 36 healthy adult male SD rats were assigned to sham group and exposure group randomly. The rats of exposure group were exposed to 1840 MHz at specific absorption rate (SAR) of 2 W/kg for 1 h/day and 7 days totally. After RF exposure, the rats were sacrificed immediately. Transmission electron microscope (TEM) of exogenous lanthanum nitrate (La3+)-tracing, albumin immunohistochemical staining and western blotting were performed to observe the permeability of BBB and the protein level of tight junction (TJ) associated protein zonula occludens-1 (ZO-1). Results: The cerebral cortex of sham group showed general normal appearance of nerve cells and endothelial cells. The lanthanum nitrate was confined to the lumen. However in exposure group, there was linear deposition of lanthanum particles between the endothelial cells and the basement membrane as well as in the brain parenchyma. Similarly, albumin immunoactivity was found around cortical microvessels. These results indicated that the permeability of BBB increased after RF exposure. Meanwhile, western blot results showed that the expression level of ZO-1 decreased slightly (t=5.438, P < 0.001) compared with the sham group. At the level of α=0.05, the difference was statistically significant. Conclusion: Exposure to radio frequency fields at 1840 MHz could increase the BBB permeability, and probably tight junction associated protein ZO-1 was involved in it. In this context, people who have been exposed to RF radiation with high SAR value for long time may suffer from high risk of BBB breakdown and CNS disorder. |
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