| 董笑克,洪明昭,韩艺凡,丁 雷,乐海波,段 颖,秦灵灵,孙 文,刘铜华,郭翔宇.骨化三醇对自身免疫性甲状腺炎大鼠T淋巴细胞亚群的影响[J].,2017,17(30):5818-5822 |
| 骨化三醇对自身免疫性甲状腺炎大鼠T淋巴细胞亚群的影响 |
| Effects of Calcitriol on T Lymphocyte Subsets of Experimental Autoimmune Thyroiditis Rats |
| 投稿时间：2017-05-21 修订日期：2017-06-15 |
| DOI：10.13241/j.cnki.pmb.2017.30.004 |
| 中文关键词: 自身免疫性甲状腺炎 骨化三醇 Th1/Th2 Th17/Treg |
| 英文关键词: Autoimmune thyroiditis Calcitriol Th1/Th2 Th17/Treg |
| 基金项目:北京市科技新星（Z141107001814011）；北京中医药大学东方医院院级课题：东方新星（DFRC2014C06） |
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| 中文摘要: |
| 摘要 目的：研究骨化三醇对自身免疫性甲状腺炎模型大鼠甲状腺功能、甲状腺过氧化物酶抗体（TPOAb）、甲状腺球蛋白抗体（TGAb）及脾脏T淋巴细胞亚群的影响。方法：20只Lewis大鼠采用高碘饮水联合猪甲状腺球蛋白皮下注射的方法建立自身免疫性甲状腺炎动物模型。造模成功后大鼠随机分为模型组和骨化三醇组。经10周灌胃后，检测各组大鼠甲状腺功能三项、甲状腺抗体，流式细胞技术检测大鼠脾脏T淋巴细胞亚群的比例。结果：①与正常组相比，模型组及骨化三醇组大鼠促甲状腺激素（TSH）明显降低，血清游离甲状腺素（FT4）及TPOAb、TGAb明显升高，差异均具有统计学意义（P＜0.01）；与模型组大鼠相比，骨化三醇组大鼠TPOAb、TGAb明显降低（P＜0.01）；② 与正常组相比，模型组大鼠表现为Th1、Th17细胞比例升高（P＜0.05），Th1/Th2、Th17/Treg比例升高（P＜0.05），与模型组相比，骨化三醇组大鼠Th1、Th17细胞比例降低（P＜0.05），Th2、Treg比例升高（P＜0.05），Th1/Th2、Th17/Treg比例降低（P＜0.01）。结论：骨化三醇可通过抑制Th1、Th17免疫亢进，改善Th1/Th2、Th17/Treg失调，改善自身免疫性甲状腺炎免疫反应。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of calcitriol on thyroid function, thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb) and spleen T lymphocyte subsets in experimental autoimmune thyroiditis rats. Methods: 20 Lewis rats raised with high iodine water combine subcutaneous injectioned porcine thyroglobulin to establish animal model of autoimmune thyroiditis. The successful model rats were divided into model group and traditional calcitriol group. After 10 weeks gavage, the thyroid function and the thyroid antibody of each group were detected, and the proportion of T lymphocyte subsets in the spleen of rats was detected by flow cytometry. Results: ①Compared with the normal group, the thyroid stimulating hormone (TSH) in model group and calcitriol group significantly decreased, serum free thyroxine (FT4) and TPOAb, TGAb increased significantly, the differences were statistically significant (P < 0.01); Compared with the model group, the TPOAb and TGAb in the calcitriol group of rats were significantly lower (P < 0.01); ②Compared with the normal group, the percentage of Th1 and Th17 cells increased (P < 0.05) in the model group, and the proportion of Th1/Th2 and Th17/Treg also increased (P < 0.05). Compared with model group, the percentage of Th1 and Th17 cells in the rats with of the calcitriol group decreased (P < 0.05), and the proportion of Th2 and Treg increased (P < 0.05), the proportion of Th1/Th2 and Th17/Treg decreased (P < 0.01). Conclusion: Ossification of three alcohol can improve the immune response of autoimmune thyroiditis by inhibiting the Th1 and Th17 hyperfunction and improving the imbalance of Th1/Th2 and Th17/Treg. |
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