| 任婧婧,李 光,刘志勤,张韵洁,谢 佳,刘 锋,戴进前,宋艳萍.RNA干扰c-maf基因表达对多发性骨髓瘤细胞凋亡的影响及机制研究[J].,2017,17(29):5637-5641 |
| RNA干扰c-maf基因表达对多发性骨髓瘤细胞凋亡的影响及机制研究 |
| Effect and Mechanism of RNA Interference of c-Maf on the Apoptosis of Multiple Myeloma Cells |
| 投稿时间：2017-03-01 修订日期：2017-03-26 |
| DOI：10.13241/j.cnki.pmb.2017.29.008 |
| 中文关键词: c-maf基因 多发性骨髓瘤 凋亡 Wnt/β-catenin信号通路 |
| 英文关键词: C-maf gene Multiple myeloma Apoptosis Wnt/β-catenin signaling pathway |
| 基金项目:陕西省科学技术研究发展计划项目（2014K11-02-03-03） |
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| 中文摘要: |
| 摘要 目的：探讨RNA干扰c-maf基因表达对多发性骨髓瘤细胞凋亡的影响及机制。方法：将人骨髓瘤细胞株NCI-H929分为正常对照组、转染siRNA Control的阴性对照组和转染siRNA c-maf基因的沉默组，转染48 h后，提取细胞中的蛋白，Western blot检测各组细胞中c-maf的蛋白表达，流式细胞仪检测细胞凋亡情况，Western blot检测凋亡相关蛋白Cleaved caspase3及Wnt/β-catenin信号通路相关蛋白β-catenin和Cyclin D1蛋白表达。结果：阴性对照组c-maf的蛋白表达与正常对照组比较差异无统计学意义(P>0.05)，沉默组c-maf的蛋白表达显著低于正常对照组(P<0.01)，而阴性对照组c-maf的蛋白表达与正常对照组比较差异无统计学意义(P>0.05)。与阴性对照组比较，沉默组的细胞凋亡率显著升高，Cleaved caspase3蛋白显著上调表达，β-catenin和Cyclin D1蛋白显著下调表达(P<0.01)。结论：RNA干扰c-maf基因表达可诱导多发性骨髓瘤细胞凋亡，其机制可能与抑制Wnt/β-catenin信号通路有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect and mechanism of RNA interference of c-Maf gene on the apoptosis of multiple myeloma cells. Methods: Human myeloma cell line NCI-H929 were divided into the normal control group, negative control group of transfected siRNA Control and silent group of siRNA c-Maf gene; cells protein were extracted after transfection for 48 h, the apoptosis was detected by flow cytometry; the expression of c-Maf, cleaved caspase3, β-catenin, Cyclin D1 protein were detected by Western blot. Results: The expression of c-Maf protein in normal and negative control group showed no significant difference (P>0.05), the expression of c-Maf protein in silent group was significantly lower than that of the normal control group (P<0.01); however, there was no significant difference in the expression of c-Maf protein between negative control group and silent group (P>0.05); compared with the negative con- trol group, the apoptotic rate of silent group was significantly increased, the expression of Cleaved Caspase3 protein was significantly up-regulated, β-catenin and Cyclin D1 protein expression were down regulated (P<0.01). Conclusion: RNA interference of c-Maf gene could induce the apoptosis of multiple myeloma cells, which might be related to the inhibition of Wnt/β-catenin signaling pathway. |
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