| 徐慧芳,张爱英,王振顺,尹成增,李 宁,林栋栋.化学发光蛋白芯片用于检测肝癌患者血清CA19-9水平的方法研究[J].,2017,17(26):5054-5057 |
| 化学发光蛋白芯片用于检测肝癌患者血清CA19-9水平的方法研究 |
| Detection of Serum CA19-9 Levels in Patients with Primary Hepatic Carcinoma by Using Chemiluminescent Protein Microarray |
| 投稿时间：2017-01-05 修订日期：2017-03-29 |
| DOI：10.13241/j.cnki.pmb.2017.26.011 |
| 中文关键词: 化学发光蛋白芯片 糖链抗原CA19-9 肝癌 诊断 |
| 英文关键词: Chemiluminescent protein microarray CA19-9 Primary hepatic carcinoma Diagnosis |
| 基金项目:国家级“十二五”科技成果转化立项项目(2015ZX10004801)子课题；国家艾滋病和病毒性肝炎等重大疾病传染病防治科技重大专项 (2012ZX10002017-007)；肝癌抗复发转移治疗临床新体系的研究和应用推广；北京市属医学科研院所公益发展改革试点项目（京医研2016-2），肝癌特异性早期预测和分期诊断分子标志物系统筛选及机制研究平台；北京市科技重大专项（D171100003117004），肝细胞癌早期诊断血清标志物研究；北京市科委课题（D121100003912002），建立乙型肝炎肝硬化无创诊断标准及模型的研究；北京市卫生系统高层次卫生技术人才培养计划（2013-3-074）；北京市教育委员会项目( KM201510025020)；北京市教委重大传染病防治协同创新中心；北京市医院管理局重点医学专业发展计划（ZYLX201311）；2017北京市肝病研究所自主课题项目，乙肝相关肝癌血清蛋白标志物蛋白芯片的研发与临床验证 |
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| 中文摘要: |
| 摘要 目的：探讨一种简单、有效、低成本、低耗时的化学发光蛋白芯片方法用于检测血清中的糖链抗原19-9 (Carbohydrate Antigen 19-9，CA19-9)，以有助于对原发性肝癌的早期辅助诊断。方法：预先在醛基芯片上包被鼠源CA19-9单克隆抗体，建立CA19-9抗体蛋白芯片，共筛选出46份肝癌血清和32份正常健康人血清，然后用蛋白芯片方法进行检测，并以化学发光成像对检测结果进行判定。结果：24份肝癌血清的CA19-9水平高于37 U/mL，22份肝癌血清的CA19-9水平低于37 U/mL；30份正常人血清CA19-9含量低于37 U/mL，2份正常人血清的CA19-9含量为37 U/mL；灵敏度为52.17 %，特异性为93.75%，ROC曲线下面积0.688 [95% CI: 0.566, 0.811] 。结论：本研究成功的建立了血清CA19-9的化学发光蛋白芯片检测方法。 |
| 英文摘要: |
| ABSTRACT Objective: To develop a simple, effective, low-cost, time saving chemiluminescent protein microarray to detect the serum CA19-9 level of patients with primary hepatic carcinoma. Methods: A protein microarray was developed for detecting CA19-9 levels in the serum samples after spotting mouse-derived CA19-9 monoclonal antibody on an aldehyde-based chip. Serum from primary hepatic carcinoma(n=46) and healthy controls(n=32) were tested by using this assay. Results: The results showed that 24 out of 46 patients with primary hepatic carcinoma had serum CA19-9 levels above 37 U/mL, and 22 out of 46 patients with primary hepatic carcinoma had serum CA19-9 levels under 37 U/mL. In healthy control, 30 out of 32 total cases were under 37 U/ml. Only two healthy controls were 37 U/mL. The sensitivity, specificity and AUC of protein microarray were 52.17%, 93.75%, 0.688 [95% CI: 0.566, 0.811]. Conclusion: A chemiluminescent protein microarray method was established for detection of CA19-9 in serum. |
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