| 邹怡君,栾 晓,柳 洋,郭菲菲,孙向荣,徐 珞.下丘脑腹内侧核Orexin-1及其受体对大鼠胃酸分泌的影响及其机制[J].,2017,17(26):5048-5053 |
| 下丘脑腹内侧核Orexin-1及其受体对大鼠胃酸分泌的影响及其机制 |
| Role of the Ventromedial Hypothalamic Orexin-1 and Orexin-1 Receptors in Regulation of Gastric Acid Secretion in Conscious Rats |
| 投稿时间：2017-03-01 修订日期：2017-03-29 |
| DOI：10.13241/j.cnki.pmb.2017.26.010 |
| 中文关键词: 下丘脑腹内侧核 orexin orexin受体 NPY Y1及Y5受体 胃酸分泌 |
| 英文关键词: Ventromedial hypothalamus Orexin Orexin-1 receptor NPY Y1 and Y5 receptors Gastric acid secretion |
| 基金项目:国家自然科学基金项目(81470815,81270460,81500414)；山东省优秀中青年科学基金项目(BS2014YY009)；青岛市科技局项目(14-2-3-3-nsh) |
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| 中文摘要: |
| 摘要 目的：探讨下丘脑腹内侧核Orexin-1及其受体对大鼠胃酸分泌的影响及其机制。方法：大鼠麻醉后侧脑室及VMH置管，大鼠分组后分别VMH注射orexin-A、[Pro34]-酪酪肽、[cPP1-7、NPY19-23、Ala31、Aib32、Gln34]胰多肽；腹腔注射SB-334867；皮下注射阿托品；侧脑室微量注射GR-231118、CGP-71683。给药结束后使用幽门结扎模型检测大鼠的胃酸分泌。结果：OXA能够促进胃酸分泌，且呈量效依赖关系。腹腔注射SB-334867能够抑制胃酸分泌，且呈量效依赖关系；SB-334867能够抑制orexin-A对胃酸分泌的促进作用；阿托品不但能够抑制胃酸分泌并且还能够完全阻断OXA的促胃酸分泌作用。侧脑室微量注射GR-231118或CGP-71683胃酸及胃液量减少，呈量效依赖关系，并且能够完全阻断OXA的促胃酸分泌作用。VMH内微量注射[cPP1-7, NPY19-23, Ala31, Aib32, Gln34]胰多肽胃酸分泌增多，且呈量效依赖关系。结论：Orexin-A能够作用于下丘脑VMH促进胃酸分泌，orexin受体、Y1和Y5受体以及迷走神经系统均参与该过程。 |
| 英文摘要: |
| ABSTRACT Objective: This study aimed to explore the Ventromedial Hypothalamic Orexin-1 and Orexin-1 Receptors in Regulation of Gastric Acid Secretion in Conscious Rats. Methods: Rats were anaesthetized and fitted with a stainless steel cannula placed just above the VMH or paracele, after random allocation orexin-A, [Pro34] - peptide YY and [CPP1-7, NPY19-23, Ala31, Aib32, Gln34]-pancreatic polypeptide were injected in the VMH; SB-334867 was intraperitoneal injection; atropine was subcutaneous injection; GR-231118 and CGP-71683 were injected in the paracele. Using pyloric ligation model, tests the effect of different drugs on rat gastric acid secretion and gastric juice volume. Results: OXA induced dose-dependent increase of gastric acid secretion; SB-334867 induced dose-dependent inhibition of gastric acid secretion. The stimulatory effect of OXA on acid secretion was inhibited by SB-334867; atropine induced dose-dependent increase of gastric acid secretion and block the effect of orexin-A on gastric acid secretion; the gastric acid secretion was inhibited by GR-231118 or CGP-71683, and GR-231118 or CGP-71683 were blocked the effect of orexin-A on gastric acid secretion; Intraventromedial hypothalamic injections of [CPP1-7, NPY19-23, Ala31, Aib32, Gln34]-pancreatic polypeptide increased gastric acid secretion. Conclusion: It is suggested that endogenous orexin-A acts on the ventromedial hypothalamus to stimulates acid secretion. This stimulatory effect is probably mediated through orexin receptor, Y1 and Y5 receptor, and the vagus nerve system. |
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