| 赵玉玺,魏 莹,陈 珍,张世鹏,张 帆.透明质酸修饰的绿原酸脂质体对U14宫颈癌荷瘤小鼠的抑制作用[J].,2017,17(26):5029-5033 |
| 透明质酸修饰的绿原酸脂质体对U14宫颈癌荷瘤小鼠的抑制作用 |
| The Anti Tumor Effect of Hyaluronic Acid Decoreted Chlorogenic Acid Liposomes on Mice Bearing Uterine Cervical Carcinoma (U14) |
| 投稿时间：2017-03-08 修订日期：2017-03-31 |
| DOI：10.13241/j.cnki.pmb.2017.26.006 |
| 中文关键词: 透明质酸 绿原酸 靶向 脂质体 肿瘤 |
| 英文关键词: Hyaluronic acid Chlorogenic acid Target Liposomes Tumor |
| 基金项目:国家自然科学基金项目（81460637） |
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| 中文摘要: |
| 摘要 目的：对透明质酸(HA)靶向绿原酸(CA)脂质体（HA-CA脂质体）进行处方筛选，以及对U14宫颈癌小鼠的抑制作用实验。方法：筛选制备HA-CA脂质体的方法，并以磷脂比、药脂比、PBS的pH为单因素考察指标通过正交实验筛选最优处方；采用透析袋法考察HA-CA的体外释放；Bal b/c小鼠右腋皮下接种U14宫颈癌瘤株，连续尾静脉注射给药14 d后，摘取瘤体称重，并计算肿瘤生长抑制。结果：采用薄膜分散法制备脂质体，最优处方为磷脂比为4:1，药脂比为1:30，PBS的pH为7.4。HA-CA脂质体与CA脂质体释放曲线基本一致，都具有一定的缓释效果。48 h时，HA-CA脂质体和CA脂质体的累计释放度分别为78.39 %、83.01 %。HA-CA脂质体对U14宫颈癌小鼠的抑瘤率为60.39%,与阳性对照组环磷酰胺相当，高于CA和CA脂质体。结论：HA-CA脂质体由于其具有主动靶向配体HA的修饰，使其抑制U14宫颈癌裸鼠的效果明显高于CA和CA脂质体。 |
| 英文摘要: |
| ABSTRACT Objective: To optimize the formulation of HA-CA liposomes and to study the anti-tumor effect of HA-CA liposomes on uterine cervical carcinoma mice. Methods: The methods of preparing HA-CA liposomes were screened, and the optimal fomulation was selected by the orthogonal design experiment with the the phospholipids/cholesterol ratio, the drug/lipids ratio and the pH value of PBS buffer was 7.4 as entrapment enfficiency was the index. The release of HA-CA liposomes was studyed by dialysis bag method.Uterine cervical carcinoma cells were inoculated subcutaneously into right axillary of Bal b/c mice, after continuous treatment of 14 d,we weighed the tumor and calculated the rate of tumor growth inhibition. Results: HA-CA liposomes were prepared by thin film hydration methods. The optimal parameters were as follows: the phospholipids/cholesterol ratio was 4:1, the drug/lipids ratio was 1: 30, the pH value of PBS buffer was 7.4. The release curve of HA-CA liposome and CA liposome was basically the same, both of which a sustained-release efficacy. The cumulative release of HA-CA liposomes and CA liposomes were 78.39% and 83.01% at 48h. The inhibition rate of HA-CA liposomes on U14 cervical cancer mice was 60.39% and significantly higher than that of positive control group, which was higher than that of CA and CA liposomes. Conclusion: HA-CA liposomes can significantly inhibit the effect of U14 cervical cancer nude mice higher than that of CA and CA liposomes owe to the modification of the active target ligand HA. |
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