| 孙英博,孙 宇,马 越,王 芳,程 翔,伊恩多.微弧氧化后锆铜铝银非晶合金的细胞相容性研究[J].,2017,17(26):5017-5021 |
| 微弧氧化后锆铜铝银非晶合金的细胞相容性研究 |
| The Cytocompatibility Study of Zr-Cu-Al-Ag Alloy Coated by Micro-arc Oxidation |
| 投稿时间：2017-02-27 修订日期：2017-03-20 |
| DOI：10.13241/j.cnki.pmb.2017.26.004 |
| 中文关键词: 微弧氧化 锆基非晶合金 细胞相容性 四唑盐 |
| 英文关键词: Micro-arc oxidation Zr-Cu-AL-Ag alloy Cytocompatibility MTT |
| 基金项目:黑龙江省教育厅科研基金项目(12531322) |
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| 中文摘要: |
| 摘要 目的：探讨微弧氧化(micro-arc oxidation，MAO)后的Zr46(Cu4.5/5.5Ag1/5.5)46Al8(at%)(本文简称Zr-Cu-Al-Ag非晶合金)的细胞相容性。方法：按照国家标准制备300 V、350 V和400 V电压MAO处理的Zr-Cu-Al-Ag非晶合金、铸态Zr-Cu-Al-Ag非晶合金以及TI6Al4V合金试件的浸提液用于培养L929细胞，阴性组的L929细胞用含10 %小牛血清的DMEM溶液培养，阳性组的L929细胞用含64 g/L苯酚和10 %小牛血清的DMEM溶液培养，通过四唑盐(MTT)比色法分析试件的细胞相容性。结果：MAO处理的Zr-Cu-Al-Ag非晶合金细胞毒性评级为0，其浸提液中的L929细胞状态良好，细胞增殖曲线呈上升趋势，三个MAO组的吸光度值高于铸态Zr-Cu-Al-Ag非晶合金组、TI6Al4V合金组和阳性对照组(P<0.05)，但与阴性对照组无明显差别(P>0.05)。结论：MAO提高了Zr-Cu-Al-Ag非晶合金表面的细胞相容性。 |
| 英文摘要: |
| ABSTRACT Objective: To study the cytocompatibility of Zr-Cu-Al-Ag alloy coated by micro-arc oxidation. Methods: Components of Zr-Cu-Al-Ag alloy coated by micro-arc oxidation in three different voltages of 300 V, 350 V and 400 V, Zr-Cu-Al-Ag alloy as cast condition and TI6Al4V alloy were made for the test.The water extracted from the components were obtained according to national standard.The L929 cells were cultivated in vitro in the extracts of these components separately. The L929 cells, cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal calf serum, served as the negative control group. And cells, cultured in Dulbecco's modified Eagle medium supplemented with 10 % fetal calf serum and 64 g/L phenol, served as the positive control group. The cytocompatibility of these components were evaluated by MTT colorimetric. Results: The cytotoxicity of Zr-Cu-Al-Ag alloy coated by micro-arc oxidation is 0 grade. Microscopy showed that the morphology of L929 cells, cultured in the extracts of Zr-Cu-Al-Ag alloy coated by micro-arc oxidation were normal. There were no significant differences between micro-arc oxidationt and negative control groups. The cell multiplication curves of micro-arc oxidation and negative control groups were nearly overlapping and in the linearity increasing trend.The OD in micro-arc oxidation groups had no significant differences with negative control group(P>0.05), but were higher than that of Zr-Cu-Al-Ag alloy as cast condition, TI6Al4V alloy and positive control groups(P<0.05). Conclusion: The cytocompatibility of Zr-Cu-Al-Ag alloy has been improved by micro-arc oxidation technique. |
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