| 彭洁颖,范茂丹,张 舜,陈岳涛,周 磊.Nogo-A蛋白在一氧化碳中毒大鼠海马组织中的动态表达[J].,2017,17(26):5007-5010 |
| Nogo-A蛋白在一氧化碳中毒大鼠海马组织中的动态表达 |
| Dynamic Expression of Nogo-A in Hippocampus of Rats after Carbon Monoxide Poisoning |
| 投稿时间：2017-03-30 修订日期：2017-04-25 |
| DOI：10.13241/j.cnki.pmb.2017.26.002 |
| 中文关键词: 一氧化碳中毒 Nogo-A 中枢神经损伤 神经生长抑制因子 |
| 英文关键词: Carbon monoxide poisoning, Nogo-A, Central nerve injury, Nerve growth inhibiting factor |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：观察一氧化碳中毒后大鼠海马组织中Nogo-A蛋白动态表达情况，探讨Nogo-A蛋白在一氧化碳中毒后神经系统损伤中的作用与影响。方法：随机数字法将雄性SD大鼠30只分为正常对照组（NC组）、CO中毒组（CO组）、CO中毒后24小时组（CO-24h）、CO中毒后48小时组（CO-48h）、CO中毒后7天组（CO-7d），每组各6只。CO气体腹腔注射染毒法建立一氧化碳中毒模型。建模后不同时间点利用免疫组织化学染色(IHC)、蛋白质免疫印迹技术检测(WB)Nogo-A蛋白在海马组织中的表达情况并分析其变化规律。结果：IHC结果表明NC、CO组、CO-24h组、CO-48h组、CO-7d组中Nogo-A蛋白平均光密度值分别为0.0928±0.0038、0.01172±0.0042、0.1452±0.0056、0.1271±0.0057、0.1088±0.0055；WB结果提示一氧化碳中毒后海马组织中Nogo-A蛋白的表达较NC组增高，在24h时表达达到高峰（P<0.05）；24h后表达开始逐步下降，至染毒第7天时，海马组织中Nogo-A蛋白的表达明显下降，但仍高于NC组（P<0.05）。结论：在本研究中，大鼠海马组织中Nogo-A蛋白表达的增高与一氧化碳中毒相关；Nogo-A蛋白表达在24h时表达达到高峰；24h后表达开始逐步下降。 |
| 英文摘要: |
| ABSTRACT Objective: To study the dynamic expression of Nogo-A in hippocampus of rats after carbon monoxide poisoning, and to explore the effect and influence of Nogo-A in the damage to nervous system after carbon monoxide poisoning. Methods: Thirty male SD rats were randomly divided into NC group(n=6), CO group(n=6), CO-24 h group(n=6), CO-48 h group(n=6), CO-7d group(n=6). The method of injection CO gas was used to establish the carbon monoxide poisoning model. Then immunohistochemical(IHC) and Western blot (WB) techniques were used to observe dynamic expression of Nogo-A in hippocampus of rats at several time intervals after carbon monoxide poisoning and to analyze its change law. Results: IHC results showed that the average optical density value of expression of Nogo-A in NC group, CO group, CO-24 h group, CO-48h group and CO-7d group were 0.0928±0.0038, 0.01172±0.0042, 0.1452±0.0056, 0.1271±0.0057, 0.1088±0.0055. WB results showed that the expression of Nogo-A in hippocampus after carbon monoxide poisoning was significantly higher than that in NC group(P<0.05), and reached the highest level at 24 h, then had a gradual recovery after 24h. The expression of Nogo-A decreased obviously, but still higher than that of NC group by day 7(P<0.05). Conclusion: In this study, the increase of expression of Nogo-A was associated with carbon monoxide poisoning. The expression of Nogo-A reached the highest level at 24h, then had a gradual recovery after 24 h. |
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