| 王 薇,张玉想,李宏山,杨 龙,王 征.血必净对脓毒症早期大鼠急性肺损伤的作用及可能机制[J].,2017,17(25):4820-4823 |
| 血必净对脓毒症早期大鼠急性肺损伤的作用及可能机制 |
| Effects and Mechanisms of Xuebijing on Early Sepsis Induced Acute Lung Injury in Rats |
| 投稿时间：2016-12-24 修订日期：2017-01-18 |
| DOI：10.13241/j.cnki.pmb.2017.25.005 |
| 中文关键词: 脓毒症 急性肺损伤 内皮素-1 诱导型一氧化氮合酶 基质金属蛋白酶-9 组织金属蛋白抑制物-1 |
| 英文关键词: Sepsis ALI ET-1 iNOS MMP-9 TIMP-1 |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：探讨血必净注射液对脓毒症早期肺组织损伤的影响及其可能作用机制，为临床脓毒症时急性肺损伤的治疗提供新思路。方法：选用健康雄性S-D大鼠30只，随机分3组，即假手术组(Sham组)、盐水组(NS组)和血必净组(XBJ组)。应用盲肠结扎穿孔法(CLP)建立脓毒症大鼠模型，术后6 h处死动物，通过HE染色光镜观察组织形态学改变及透射电镜观察肺组织超微结构，测定各组肺组织湿干重比，采用RT-PCR检测肺组织内皮素-1(ET-1)、诱导型一氧化氮合酶(iNOS)、基质金属蛋白酶-9(MMP-9)、组织金属蛋白酶抑制物-1(TIMP-1) mRNA的表达。结果：XBJ组肺泡及肺间质水肿和内皮细胞超微结构改变均较NS组明显减轻。术后6 h，NS组大鼠肺组织湿干重比(W/D)显著高于Sham组(5.37±0.12 vs 4.33±0.06，P<0.01)，XBJ组肺组织W/D较NS组显著降低(4.67±0.09 vs 5.37±0.12，P<0.05)。此外，XBJ组大鼠肺组织ET-1 (0.511±0.111 vs 0.705±0.122，P<0.01)、iNOS(0.456±0.075 vs 0.548±0.098，P<0.05)，MMP-9(0.617±0.079 vs 0.732±0.131，P<0.05)、TIMP-1(0.438±0.043vs 0.515±0.049，P<0.01) mRNA的表达均较NS组明显降低，而NS组大鼠肺组织ET-1 (0.705±0.122 vs 0.400±0.033，P<0.01)、iNOS(0.548±0.098 vs 0.334±0.027， P<0.01)、MMP-9(0.732±0.131 vs 0.352±0.061，P<0.01)、TIMP-1mRNA(0.515±0.049 vs 0.365±0.068，P<0.01)水平均较Sham组明显升高。结论：血必净治疗对脓毒症大鼠的肺组织损伤有一定的保护作用，可能与其降低肺组织ET-1、iNOS、MMP-9、TIMP-1的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects and mechanisms of Xuebijing injection on early sepsis induced acute lung injury and explore a new therapy. Methods: Thirty healthy male Sprague-Dawley rats were randomly divided into 3 groups: Sham group(n=10), NS group (NS 4mL/kg n=10), Xuebijing Group(Xuebijing 4mL/kg n=10). Cecal ligation and puncture (CLP) was used to duplicate se- vere sepsis model. At 6h after CLP, the rats were sacrificed, the lungs were resected and histopathological characteristic was observed by transmission electron microscopy technique. The change of the lung wet/dry (W/D) weight ratio were studied. Meanwhile, the lungs were resected for detection of ET-1, iNOS, MMP-9, TIMP-1 mRNA with reverse transcription-polymerase chain raction (RT-PCR). Results: The changes of pulmonary alveoli and the interstitial edema as well as lung tissue in Xuebijing group were better than those of NS group. The change of lung wet/dry (W/D) weight ratio in septic rats showed was significantly increased at 6 h after CLP (vs Sham group: 5.37±0.12 vs 4.33±0.06, P<0.01). The lung W/D was significantly decreased in XBJ group (vs NS group: 4.67±0.09 vs 5.37±0.12, P<0.05). The expressions in XBJ group lung tissue of ET-1(0.511±0.111 vs 0.705±0.122, P<0.01), iNOS(0.456±0.075 vs 0.548±0.098， P<0.05)、MMP-9(0.617±0.079 vs 0.732±0.131, P<0.05), TIMP-1( 0.438±0.043 vs 0.515±0.049, P <0.01) mRNA were significantly de- creased than those in NS group. And the expressions of ET-1(0.705±0.122vs 0.400±0.033, P<0.01), iNOS(0.548±0.098 vs 0.334±0.027, P<0.01), MMP-9(0.732±0.131 vs 0.352±0.061, P<0.01), TIMP-1(0.515±0.049 vs 0.365±0.068, P<0.01) mRNA in NS group were significantly increased compared with those in the sham group. Conclusion: Xuebijing could protect against sepsis induced acute lung injury, which might be related with the decrease of ET-1, iNOS, MMP-9 and TIMP-1 mRNA expressions. |
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