| 史纪元,易 智,刘宗智,姬 乐,刘慧通.不同程度骨关节炎中Wnt/β-catenin信号通路、OPN和MMP-13的相关性的研究[J].,2017,17(24):4639-4644 |
| 不同程度骨关节炎中Wnt/β-catenin信号通路、OPN和MMP-13的相关性的研究 |
| Correlation Analysis of Wnt/β-catenin Signaling Pathway, OPN and MMP-13 in Varying Degrees of Osteoarthritis |
| 投稿时间：2016-11-01 修订日期：2016-11-20 |
| DOI：10.13241/j.cnki.pmb.2017.24.009 |
| 中文关键词: 骨关节炎 Wnt/β-catenin信号通路 OPN MMP-13 相关性 |
| 英文关键词: Osteoarthritis (OA) Wnt/β-catenin signaling pathway OPN MMP-13 Correlation |
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| 中文摘要: |
| 摘要 目的：研究不同严重程度骨关节炎模型兔的软骨细胞中Wnt/β-catenin信号通路、骨桥蛋白（Osteopontin, OPN）和基质金属蛋白酶13（Matrix metalloproteinase 13, MMP-13）的相关性。方法：将40只新西兰大耳白兔随机分成4组，通过注射不同浓度木瓜蛋白酶处理，而构建患有不同程度骨关节炎的兔模型。利用Real-Time PCR法和ELISA法分别检测β-catenin、OPN、MMP-13以及Ⅱ型胶原和蛋白聚糖的mRNA和蛋白的水平。结果：利用不同浓度的木瓜蛋白酶成功建立OA兔模型，通过Mankin评分可分成轻度、中度和重度三级。在不同分级的OA模型兔中，β-catenin、OPN、MMP-13的浓度均高于正常对照组，而Ⅱ型胶原和蛋白聚糖较正常对照组均下降(P<0.05)；且随着OA严重程度增加，结果具有一致性(P<0.05)。结论：Wnt/β-catenin信号通路可调节OPN的表达，进而影响到MMP-13的表达，最终对骨关节炎的发生产生影响。 |
| 英文摘要: |
| ABSTRACT Objective: To analysis the correlation of Wnt/β-catenin signaling pathway, OPN and MMP-13 in rabbit model with different degree of osteoarthritis (OA). Methods: Forty New Zealand white rabbits were randomly divided into four groups of 10 each, then we constructed the rabbit model with different degree of osteoarthritis (OA) by different concentrations of papain. The mRNA and protein levels of β-catenin, OPN, MMP-13, type Ⅱcollagen and proteoglycan were detected by Real-Time PCR and ELISA, respectively. Results: We have successfully established OA rabbit model by different concentrations of papain, and these OA rabbits model could be divided into mild, moderate and severe three level by Mankin scoring system. chondrocytes. Compared with the normal control group，the protein levels of β-catenin，OPN, and MMP-13 in the osteoarthritis were significantly increased, while the protein levels of type II collagen and proteoglycan were significantly decreased (P<0.05). With the increasing severity of OA, the results were consistent (P<0.05). Conclusion: These results indicated that Wnt/β-catenin signaling pathway might regulate the expression of OPN, thereby affecting the expression of MMP-13, and ultimately have an impact on the occurrence of osteoarthritis. |
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