| 王三虎,满荣勇,高细强,杨长顺,邓 欢,朱 琳,郑小飞,周细国.脂肪干细胞的诱导分化及其来源的研究[J].,2017,17(24):4606-4609 |
| 脂肪干细胞的诱导分化及其来源的研究 |
| Research on the Induced Differentiation of Adipose Derived Stem Cells and Its Origin |
| 投稿时间：2016-12-11 修订日期：2016-12-30 |
| DOI：10.13241/j.cnki.pmb.2017.24.002 |
| 中文关键词: 脂肪干细胞 脂肪组织 成骨分化 成脂分化 脂肪干细胞的起源 |
| 英文关键词: Adipose-derived stem cells Adipose tissue Adipogenesis differentiation Osteogenesis differentiation Origin of adi- pose-derived stem cells |
| 基金项目:怀化市科技局项目（S2015F102P010） |
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| 中文摘要: |
| 摘要 目的：通过组织块培养法得到脂肪干细胞（adipose-derived stem cells, ADSCs），探讨其诱导分化潜能，并初步研究ADSCs的来源。方法：用脂肪组织块培养法培养原代人ADSCs。第三代ADSCs进行成脂和成骨诱导分化，分别用油红O和茜素红S染色进行鉴定。脂肪组织块培养七天后取脂肪组织进行Hematoxylin-eosin Staining（HE）染色观察ADSCs组织分布。结果：用脂肪组织块培养法成功培养出原代人ADSCs。ADSCs传代到第8代，依然保持着良好的增殖能力和细胞形态。ADSCs能成功诱导成脂肪细胞和骨细胞。通过对培养七天后的脂肪组织块进行HE染色，发现ADSCs主要分布在脂肪组织的间质血管和结缔组织周围。结论：用脂肪组织块培养出来的ADSCs具有成脂和成骨分化的潜能。ADSCs主要定位于间质血管和结缔组织周围。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the induction and differentiation potential of ADSCs by tissue culture method, and to prelim- inary study on the origin of ADSCs. Methods: Using adipose tissue culture method to culture human ADSCs. The third generation of ADSCs for the adipogenic and osteogenesis differentiation, and staining by oil red O and alizarin red S. HE staining was performed after the seventh day culture of adipose tissue. Results: The primary human ADSCs were successfully cultured with adipose tissue culture method. ADSCs cultured to the eighth generation, still maintained a good proliferation ability and cell morphology. ADSCs can be success- fully induced into adipose cells and bone cells. ADSCs were mainly distributed around the mesenchymal vascular and connective tissue, by HE staining of adipose tissue after seven days of culture. Conclusion: The cells that were cultured with adipose tissue have the poten- tial to adipogenic and osteogenesis differentiation. The ADSCs were mainly distributed around the mesenchymal vascular and connective tissue. |
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