| 邱怀娜,查贺飞,曲佳乐,王 梅,冯源发,黄 俊,杨 权.IL-27增强TNF-α介导上调的炎症因子的表达[J].,2017,17(23):4412-4415 |
| IL-27增强TNF-α介导上调的炎症因子的表达 |
| Interleukin-27 Enhances TNF-α-mediated Upregulation of Inflammatory Cytokine and Chemokine |
| 投稿时间：2017-03-02 修订日期：2017-03-25 |
| DOI：10.13241/j.cnki.pmb.2017.23.003 |
| 中文关键词: 血管炎症 细胞因子 趋化因子 人冠状动脉内皮细胞 |
| 英文关键词: Vascular inflammation Cytokine Chemokine Human coronary artery endothelial cells |
| 基金项目:国家自然科学基金项目（81401309）；广东省科技计划项目（2016A020215164） |
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| 中文摘要: |
| 摘要 目的：动脉粥样硬化是慢性炎症疾病，免疫细胞及炎症因子在其发病过程中起重要调节作用。本研究在于探讨IL-27对血管内皮细胞的直接作用。方法：采用ELISA方法，研究IL-27和TNF-α对人冠状动脉内皮细胞产生炎症相关细胞因子和趋化因子的作用。结果：IL-27显著增强TNF-α介导上调的炎症相关细胞因子IL-6和趋化因子CCL5的表达。 IL-6 和CCL5的表达受特异性信号分子抑制剂显著抑制。结论：人冠状动脉内皮细胞诱导释放IL-6 和CCL5可能受JNK, p38MAPK和NF-κB通路差异调控。这些结果为阐明IL-27与TNF-α在动脉粥样硬化血管炎症发生中的作用提供重要生物化学基础。 |
| 英文摘要: |
| ABSTRACT Objective: Immune cells and inflammatory mediators play important roles in the development of atherosclerotic vascu- lar inflammation. This study focuses on the direct effect of IL-27 on human coronary artery endothelial cells. Methods: In this study, the effects of IL-27 and TNF-α on inflammatory cytokines and chemokines were investigated. Results: IL-27 could significantly enhance the TNF-α-mediated upregulation of inflammatory cytokine IL-6 and chemokines CCL5 from human coronary artery endothelial cells. The release of IL-6 and CCL5 was significantly suppressed by specific signaling molecule inhibitors. Conclusion: The induction of these me- diators from the human coronary artery endothelial cells could be differentially regulated by the c-Jun N-terminal kinase, p38 mitogen-activated protein kinase, and nuclear factor-κB pathways. These results provided new insights into the effect of IL-27 on the TNF-α medi- ated activation of human coronary artery endothelial cells in atherosclerotic vascular inflammation. |
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