| 武玉芝,郭艳芹,俞春雷,杨晓帆,毕鹏翔,安 宁,曹文慧.Neuroligin对SH-SY5Y细胞增殖和凋亡的影响[J].,2017,17(22):4246-4249 |
| Neuroligin对SH-SY5Y细胞增殖和凋亡的影响 |
| Effects of Neuroligin on the Proliferation and Apoptosis of SH-SY5Y Cells |
| 投稿时间：2015-12-30 修订日期：2016-01-25 |
| DOI：10.13241/j.cnki.pmb.2017.22.010 |
| 中文关键词: Neuroligin 神经元 细胞凋亡 PSD-95 Bax |
| 英文关键词: Neuroloigin Neuron Apoptosis PSD-95 Bax |
| 基金项目:国家自然科学基金项目（81141080）；黑龙江省卫生厅科研课题（2012-305） |
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| 中文摘要: |
| 摘要 目的：探讨Neuroligin(NLG)对人神经母细胞瘤SH-SY5Y增殖和凋亡的影响。方法：体外培养SH-SY5Y细胞24 h后，分别用浓度为50，100，200 μmol/L 的NLG siRNA转染SH-SY5Y细胞并共孵育24 h，然后采用噻唑蓝( MTT) 法检测不同浓度NLG siRNA对SHSY5Y细胞增殖率的影响，以及Real-time PCR 法检测SH-SY5Y细胞中凋亡基因Bax、Bcl-2、Bcl-xL和caspase-9基因表达水平的变化。结果：与空白对照组及siRNA阴性对照组相比，转染NLG siRNA后SH-SY5Y细胞增殖率显著下降，细胞凋亡相关基因被激活，导致细胞凋亡。结论：NLG对神经元细胞具有保护作用，抑制神经细胞凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To study effects of neuroligin(NLG) on the proliferation and apoptosis in human neuroblastoma SH-SY5Y cells. Methods: The SH-SY5Y cells were cultured in vitro for 24 hours,and then transfected with NLG siRNA at dose of 50, 100, 200 μmol/L, respectively. MTT procedure was used to detect the cell proliferation, and expression levels of apoptosis gene including Bax or Bcl-2 and Bcl-xl were measured by RT-PCR. Results: Compared to control groups proliferation of SH-SY5Y cells were distinctly inhibited after NLG siRNA transfection accompany with a dose-dependent, which was caused by activation of apoptosis. Conclusion: NLG protect neuron by inhibiting apoptosis. |
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