| 王秀新,罗茂华,贾洪涛,孙 涛,杨 华,李玉琼.miR-139-5p在前列腺癌患者外周血中的表达及临床意义[J].,2017,17(19):3690-3692 |
| miR-139-5p在前列腺癌患者外周血中的表达及临床意义 |
| Expression and Clinical Significance of miR-139-5p in Peripheral Blood of Patients with Prostate Cancer |
| 投稿时间：2016-12-27 修订日期：2017-01-23 |
| DOI：10.13241/j.cnki.pmb.2017.19.022 |
| 中文关键词: miR-139-5p 前列腺癌 外周血清 肿瘤标志物 |
| 英文关键词: miR-139-5p Prostate cancer Peripheral blood Tumor markers |
| 基金项目:湖北省青年科技人才基金资助项目(QJX2008-38） |
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| 中文摘要: |
| 摘要 目的：研究miR-139-5p在前列腺癌患者外周血中的表达及临床意义。方法：收集2015年4月至2016年9月于我院进行诊治的65例前列腺疾病患者和20例男性健康志愿者外周血样本，使用RT-qPCR方法检测各组miR-139-5相对表达量，统计分析前列腺癌患者外周血miR-139-5p水平与临床特征相关性，使用ROC曲线分析外周血miR-139-5p诊断前列腺癌的临床价值。结果：与良性增生组(n=15)患者和对照组(n=20)健康志愿者相比，前列腺癌组(n=50)患者外周血中miR-139-5p相对表达量均显著升高(P均<0.05)。中高分化、转移癌、Gleason评分高危前列腺癌患者外周血miR-139-5p相对表达量显著高于低分化、原位癌和Gleason评分中危的前列腺癌患者(P均<0.05)。外周血miR-139-5p在区分前列腺癌和良性前列腺增生或健康人中特异性和敏感性均较高，ROC曲线下面积为0.942(95%CI：0.0.785~0.971)。结论：miR-139-5p在50例前列腺癌患者外周血中呈高表达，或可作为非侵入性前列腺癌诊断标志物。 |
| 英文摘要: |
| ABSTRACT Objective: To study the expression and clinical significance of miR-139-5p in peripheral blood of patients with prostate cancer. Methods: Patients of prostate disease (n=65) and cases of male healthy volunteers (n=20) from April 2015 to September 2015 were included in the study. RT-qPCR method was used to detect the relative expression of miR-139-5p in peripheral blood of all the cases. The ROC curve was used to analyze the clinical value of peripheral blood miR-139-5p in the diagnosis of prostate cancer. Results: Compared with the hyperplasia of prostate group (n=15) and the control group (n=20), relative expression miR-139-5p in the peripheral blood of prostate cancer group (n=50) was significantly higher (P<0.05). The miR-139-5p relative expression levels in peripheral blood of prostate cancer patients with high differentiation, metastatic carcinoma and Gleason score at high risk for were significantly higher than those with low differentiation, in situ cancer and Gleason scores(P<0.05). The peripheral blood miR-139-5p had higher specificity and sensitivity in distinguishing prostate cancer from benign prostatic hyperplasia and healthy people. Conclusion: miR-139-5p in peripheral blood of patients with prostate cancer was highly expressed and could be used as a noninvasive diagnostic markers of prostate cancer. |
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