| 朱宗成,盛晓东,周建龙,范 韬,金骁琦.ESM-1预处理小鼠骨髓间充质干细胞的实验研究[J].,2017,17(18):3451-3455 |
| ESM-1预处理小鼠骨髓间充质干细胞的实验研究 |
| Experimental Study on ESM-1 Pretreatment with MSCs of Mice |
| 投稿时间：2016-10-18 修订日期：2016-11-10 |
| DOI：10.13241/j.cnki.pmb.2017.18.011 |
| 中文关键词: 内皮细胞特异性分子-1 骨髓间充质干细胞 血管内皮生长因子 碱性磷酸酶 |
| 英文关键词: Endothelial cell-specific molecule 1 Bone marrow-derived mesenchymal stem cells Vascular endothelial growth factor Alkaline phosphatase |
| 基金项目:常熟市科技局和卫生局资助项目（CS201423） |
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| 中文摘要: |
| 摘要 目的：探讨小鼠骨髓间充质干细胞（Bone marrow-derived mesenchymal stem cells，MSCs）经不同浓度的内皮细胞特异性分子-1（Endothelial cell-specific molecule 1，ESM-1）干预后，对其分泌的细胞因子的影响。方法：选择不同浓度的ESM-1和不同干预时间，对MSCs进行预处理；分析其分泌的VEGF、ALP等代表细胞活性的细胞因子含量改变情况；明确ESM-1预处理MSCs所需的最佳干预浓度和最佳干预时间。结果：通过不同ESM-1浓度干预MSCs，其中H（0.55 μg/mL ESM-1）组条件培养液中VEGF和ALP含量同最高，分别为（82.500 ±3.307）和（0.228 ±0.020）pg/mL，差异有统计学意义，P<0.001。选择0.55 μg/mL ESM-1对MSCs进行不同时间的干预，结果显示d（3 h）组条件培养液中VEGF含量最高，g（4.5 h）组ALP含量同最高，分别为（69.112 ±3.618）和（0.352 ±0.030）pg/mL，差异有统计学意义，P<0.001。结论：不同干预因子预处理MSCs所需的最佳浓度和预处理时间不一。ESM-1对MSCs的最佳干预浓度为0.55 μg/mL。不同组织细胞的细胞因子的表达和所受到的影响因素也不相同。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate whether the secretion of Cytokines by mouse MSCs were increased after pretreated by ESM-1 with different concentration. Methods: The MSCs were pretreated by different concentration of ESM-1 and different intervention time，then to analyze the cytokines secretion of VEGF and ALP on behalf of cell activity changes of MSCs，in order to make clear of ESM-1 pretreatment intervention concentration and optimal intervention time needed for MSCs. Results: Through the different concentration of ESM-1 to pretreat of MSCs, VEG and ALP concentration in H (0.55 μg/mL ESM-1) group of conditioned medium also reached the highest value, respectively (82.500±3.307) and (0.228±0.020)pg/mL, and the difference of which was statistically significant, P<0.001. Choosing 0.55 μg/mL ESM-1 to intervene MSCs of different time, research shows that the concentration of VEGF is highest in the medium of d (3 h) group and the concentration of ALP is highest in the medium of g (4.5 h) group, respectively (69.112 ±3.618) and (0.352 ±0.030) pg/mL, and the difference was statistically significant, P<0.001. Conclusion: The optimum concentration and pretreatment time of different intervention factors required is different. The best intervention concentration of ESM-1 is 0.55 μg/mL, which pretreatment for MSCs. The expression of cytokines in different tissue cells and the influence of the process are not the same. |
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