| 张志斌,席云峰,霍龙伟,任贺庄,蒋占鑫,席俊峰.非小细胞肺癌CD166+干细胞的分离和鉴定[J].,2017,17(16):3035-3038 |
| 非小细胞肺癌CD166+干细胞的分离和鉴定 |
| CD166+ Isolation and Identification of Stem Cells in the Non-small Cell Lung Cancer |
| 投稿时间：2017-02-27 修订日期：2017-03-30 |
| DOI：10.13241/j.cnki.pmb.2017.16.009 |
| 中文关键词: 非小细胞肺癌 CD166 肿瘤干细胞 |
| 英文关键词: Non-small Cell Lung Cancer CD166 Cancer stem cell |
| 基金项目:陕西省科技厅科研基金项目（2012K16-09-03） |
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| 中文摘要: |
| 摘要 目的：分选人非小细胞肺癌中的CD166+细胞，并验证其肿瘤干细胞的特性。方法：收集手术切除的15例非小细胞癌组织标本以及患者的临床资料，并通过流式细胞术对组织中CD166+的表达水平进行检测，对CD166+细胞进行原代培养，对比CD166+细胞和CD166-细胞的成球能力和集落形成能力；并将CD166+细胞和CD166-细胞注射到小鼠体内，比较其体内肿瘤形成能力。结果：在15例临床样本中均检测到CD166+细胞，CD166阳性表达率最高为12.1%，最低为0.3%。CD166的高表达与肿瘤TNM分期、肿瘤转移、以及肿瘤分型密切相关(P均<0.05)。CD166+细胞的成球形成能力和集落形成能力显著的高于CD166-细胞，且CD166+细胞在裸鼠体内肿瘤形成能力显著高于CD166-细胞(P均<0.05)。结论：非小细胞肺癌CD166+细胞具有肿瘤干细胞的特征，CD166在非小细胞肺癌的发生发展中可能具有重要的作用。 |
| 英文摘要: |
| ABSTRACT Objective: To study the separation of CD166+ cells in human non-small cell lung cancer, and verify the characteristics of cancer stem cells. Methods: To collect surgical removal of the 15 patients with non-small cell carcinoma tissue samples and clinical data, and by flow cytometry to detect CD166+ expression levels in the organization, on the original CD166+ cells in the original generation training, contrast and CD166+/- cells in vitro self-renewal ability; CD166+ cells and CD166- cells injected into mice, differences in the ability of tumor formation in vivo. Results: CD166+ cells were detected in 15 cases of clinical samples, CD166 positive expression rate was as high as 12.1%, the lowest was 0.3%. CD166 high expression was closely related to tumor TNM stage, tumor metastasis and tumor classification (P<0.05). CD166+ cell clone formation ability significantly higher than that of CD166- cells, and CD166+ cells in nude mice tumor formation ability was significantly higher than that of CD166- cells in the body. Conclusion: Non-small cell lung cancer CD166+ cells has the characteristics of cancer stem cells, prompt CD166 in non-small cell lung cancer development may play an important role. |
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