柴晓宇,许慧莹,刘钟桧,易 亮,刘新民.氯化钴对原代大鼠肺动脉成纤维细胞的影响[J].,2017,17(15):2801-2804 |
氯化钴对原代大鼠肺动脉成纤维细胞的影响 |
Effect of Cobalt Chloride on Pulmonary Arterial Fibroblasts of Rat |
投稿时间:2016-12-09 修订日期:2016-12-29 |
DOI:10.13241/j.cnki.pmb.2017.15.001 |
中文关键词: 氯化钴 肺动脉成纤维细胞 增殖 PI3K |
英文关键词: Cobalt chloride Pulmonary arterial fibroblasts Proliferation PI3K |
基金项目:国家自然科学基金项目(81270114) |
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中文摘要: |
摘要 目的:探究氯化钴对于原代大鼠肺动脉成纤维细胞(PAF)的增殖、迁移、表型转化作用的影响。方法:分离培养大鼠肺动脉成纤维细胞,利用氯化钴刺激PAF细胞,并通过MTT、细胞划痕、Transwell、表型转化标志蛋白测定以及PI3K/Akt信号通路蛋白的变化研究氯化钴对PAF的影响。结果:MTT结果显示,与对照组相比,氯化钴可以抑制大鼠肺动脉成纤维细胞的增殖活性(P<0.001),并呈浓度依赖性。划痕实验及Transwell实验提示较高氯化钴(200 μmol?L-1)处理PAF细胞后可以抑制细胞迁移。随浓度增加,氯化钴可以抑制PAF的P110α、p-Akt蛋白表达。结论:氯化钴对于体外培养的原代大鼠肺动脉成纤维细胞的增殖、迁移和表型转化特性有一定的抑制作用,这可能与氯化钴抑制PI3K/Akt信号通路的表达有关。 |
英文摘要: |
ABSTRACT Objective: Study the effects of cobalt chloride on the proliferation, migration and phenotype transformation on the pri- mary pulmonary artery fibroblasts (PAF) from rat. Methods: Using cobalt chloride to stimulate the established PAF. Value the effects of cobalt chloride on PAF by MTT, cell scratch, Transwell, phenotype marker protein expression and PI3K/Akt signaling pathway proteins expressions. Results: Compared with the control group, MTT results showed that cobalt chloride could decrease the proliferation activity of PAF in a concentration-dependent manner. Scratch and Transwell assays showed that higher concentrations of cobalt chloride could inhibit the migration of PAF. Cobalt chloride could decrease the protein expressions of P110α and p-Akt in a concentration-dependent manner. Conclusion: Cobalt chloride could inhibit the proliferation, migration and phenotype conversion characteristics of PAF by af- fecting the expression of PI3K/Akt signaling pathway which might involve in the regulation of cell behaviors. |
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