| 阴弯弯,李项瑞,闫晋琪,李 楠,张二飞,涂 可,闫汝虎,张 莉,侯立朝,雷迎峰.高氧抑制LPS/ATP诱导的骨髓源性巨噬细胞的焦亡[J].,2017,17(12):2232-2235 |
| 高氧抑制LPS/ATP诱导的骨髓源性巨噬细胞的焦亡 |
| Hyperoxia Inhibits LPS/ATP-Induced Pyroptosis of Bone Marrow Derived Macrophages |
| 投稿时间：2016-10-23 修订日期：2016-11-07 |
| DOI：10.13241/j.cnki.pmb.2017.12.008 |
| 中文关键词: 氧气 骨髓源性巨噬细胞 白细胞介素-1β 焦亡 |
| 英文关键词: Oxygen Bone marrow derived macrophages IL-1β Pyroptosis |
| 基金项目:国家自然科学基金项目(81171839) |
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| 中文摘要: |
| 摘要 目的：研究不同浓度的氧气在LPS/ATP诱导的骨髓源性巨噬细胞焦亡中的作用。方法：提取C57BL/6小鼠的骨髓源性巨噬细胞，用1 μg/ml脂多糖(LPS)刺激细胞24 h，用5 mM 三磷酸腺苷(ATP)刺激细胞4 h，酶联免疫吸附测定法(ELISA)检测细胞培养上清液中IL-1β水平的变化。用5 mM ATP刺激细胞后，给予细胞40 %、60 %和100 %的氧气处理1.5 h，ELISA检测细胞培养上清液中IL-1β水平的变化。结果：1 μg/mL LPS和5 mM ATP先后刺激下，骨髓源性巨噬细胞培养上清液中IL-1β的水平明显升高(P<0.001)，用caspase-1特异性抑制剂AC-YVAD-CMK刺激骨髓源性巨噬细胞后IL-1β水平明显降低(P<0.001)。5 mM ATP刺激之后给予细胞不同浓度的氧气干预1.5 h后，细胞培养上清液中IL-1β的水平明显下降。结论：高氧抑制LPS/ATP诱导的骨髓源性巨噬细胞的焦亡。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of different concentrations of oxygen in LPS/ATP induced pyroptosis of bone marrow derived macrophages. Methods: To extract the bone marrow derived macrophages of C57BL/6 mice and cells were primed with 1 μg/ml LPS for 24 h, and then treated with 5 mM ATP for 4 h. IL-1β 1evels in the cell supernatants were determined using a commercially available mouse IL-1β ELISA kit. Give cells 40 %, 60 % or 100 % oxygen inhalation after 5 mM ATP stimulation and determine the IL-1β 1evels in the cell supernatants. Results: ELISA test showed IL-1β 1evels in the bone marrow derived macrophages supernatants were greatly increased after the stimulation of 1 μg/ml LPS and 5 mM ATP (P<0.001) and were declined after the stimulation of caspase-1 inhibitor AC-YVAD-CMK (P<0.001). ELISA test also showed IL-1β 1evels in the bone marrow derived macrophages supernatants were notably declined after the 1.5 hours stimulation of 40 %, 60 % and 100 % oxygen inhalation followed the stimulation of 5 mM ATP. Conclusion: Hyperoxia inhibits the pyroptosis of bone marrow derived macrophages. |
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