| 崔莹莹,袁学华,李 柯,柯盈月,王字卉,王小娟,李洪涛.五苓胶囊对前列腺癌循环miRNA-141表达的影响[J].,2017,17(11):2140-2143 |
| 五苓胶囊对前列腺癌循环miRNA-141表达的影响 |
| Effects of Five Ling Capsules on the Circulating miRNA-141 Expression of Patients with Prostate Cancer |
| 投稿时间：2016-09-15 修订日期：2016-10-14 |
| DOI：10.13241/j.cnki.pmb.2017.11.037 |
| 中文关键词: miRNA-141 前列腺癌 荧光定量PCR法 五苓胶囊 |
| 英文关键词: miRNA-141 Prostate cancer Fluorescence quantitative PCR method Five ling capsules |
| 基金项目:湖北省自然科学基金项目(2001ABA263) |
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| 中文摘要: |
| 摘要 目的：采用荧光定量PCR法检测前列腺癌循环miRNA-141表达，并研究五苓胶囊对其影响。方法：选取我院泌尿外科收治的前列腺癌患者20例，随机分为A、B两组，各10例；A组给予常规治疗，B组在常规治疗基础上给予加用五苓胶囊治疗。选取同期住院前列腺增生患者20例，正常对照组为同时期体检健康者20例。采用荧光定量PCR法检测正常对照组、前列腺增生组以及前列腺癌组血清miRNA-141的表达情况，以及前列腺癌组治疗前TPSA(前列腺特异性抗原)含量。结果：前列腺癌组的血清miRNA-141的表达水平为正常对照组的(11.22±8.19)倍，明显高于前列腺增生组(1.65±1.73)和正常对照组，差异有统计学意义(P＜0.05)；前列腺增生组和正常对照组比较无明显差异(P＞0.05)； 前列腺癌组血清miRNA-141的表达水平与TPSA水平呈正相关关系，相关系数r=0.753，有统计学意义(P＜0.05)。 治疗后，前列腺癌A组与B组的血清miRNA-141的表达水平均有所改善，与A组(5.82±4.51)相比较，B组的miRNA-141的表达水平(1.93±0.67)明显降低，差异有统计学意义(P＜0.05)。结论：前列腺癌患者血清中miRNA-141表达水平明显升高，且与TPSA水平呈正相关关系，五苓胶囊能够降低miRNA-141表达。 |
| 英文摘要: |
| ABSTRACT Objective: To detect the circulating miRNA-141 expression of prostate cancer by fluorescent quantitative PCR method, and study the effects of five ling capsules on it. Methods: 20 patients with prostate cancer who were treated in our hospital were selected and randomly divided into group A and B with 10 cases in each group; the patients in group A were given conventional treatment, pa- tients in the group B were given 3 tablets/time of five ling capsules on the base of conventional treatment, 2 times a day oral, treated 1 month in a row. 20 patients with prostate gland hyperplasia who were treated in our hospital during this period were selected, 20 cases in the normal control group were the physical health person for the same period. The serum miRNA-141 expression was detected by fluo- rescence quantitative PCR method, and TPSA content of prostate cancer treatment group (prostate specific antigen) before treatment was also detected. Results: The serum miRNA-141 expression of prostate cancer group was (11.22±8.19) times of the normal control group, which was significantly higher than the prostate gland hyperplasia group (1.65±1.73) and normal control group (1)(P<0.05); no statisti- cally significant difference was found between the prostate hyperplasia group and normal control group(P>0.05); the serum miRNA-141 expression of prostate cancer group was positively correlated with the TPSA level, the correlation coefficient r=0.753(P<0.05). After treatment, the serum miRNA-141 expression of prostate cancer group A and group B were improved, compared with group A (5.82±4.51), the miRNA-141 expression of group B (1.93±0.67) significantly decreased(P<0.05). Conclusion: The serum miRNA-141 expres- sion of prostate cancer patients was significantly increased, and was positively correlated with the TPSA level, five ling capsule could sig- nificantly reduce the miRNA-141 expression of prostate cancer patients. |
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