| 杨瑞鑫,高 立,黄 露,李玉骞,高国栋.白藜芦醇对6-羟基多巴所致SN4741细胞损伤的保护作用和机制研究[J].,2017,17(11):2020-2023 |
| 白藜芦醇对6-羟基多巴所致SN4741细胞损伤的保护作用和机制研究 |
| Resveratrol Protects the Impairment of SN4741 Cells Induced by 6-Hydroxy Dopamine |
| 投稿时间：2016-10-19 修订日期：2016-11-08 |
| DOI：10.13241/j.cnki.pmb.2017.11.005 |
| 中文关键词: 白藜芦醇 DJ-1 帕金森病 氧化应激 线粒体功能 |
| 英文关键词: Resveratrol DJ-1 Parkinson's Disease Oxidative Stress Mitochondrial function |
| 基金项目:国家自然科学基金青年科学基金项目（81401044）；陕西省自然科学基础研究计划项目（2014JQ2-8052） |
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| 中文摘要: |
| 摘要 目的：探讨白藜芦醇对6-羟基多巴引起的细胞损伤的内在保护机制。方法：以SN4741细胞系为实验对象，分为对照组、6-羟基多巴处理组和白藜芦醇预处理、6-羟基多巴处理组组。MTT法测定细胞活性。Western blot检测细胞内DJ-1表达水平。ROS检测反映细胞的氧化应激水平和线粒体损伤情况。线粒体膜电位检测反映细胞线粒体功能。结果：白藜芦醇可以剂量依赖性方式提高6-OHDA诱导的SN4741细胞的存活率。白藜芦醇预处理显著逆转6-OHDA诱导的SN4741细胞DJ-1水平的下降，降低6-OHDA引起的氧化应激水平和线粒体损伤。结论：白藜芦醇预处理能够保护6-羟基多巴所致的SN4741细胞损伤，可能与提高DJ-1的表达，减轻细胞内的氧化应激水平，改善线粒体功能有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the mechanism underlying the protection effect of resveratrol against 1. cellular impairment induced by 6-hydroxy dopamine. Methods: SN4741 cells were divided into three groups: the control group, the 6-hydroxy dopamine treated group and the resveratrol pre-treated group. The cellular viability were measured by MTT assay. DJ-1 level were assessed by Western-Blot assay. ROS level, and MMP were detected to reflect cellular oxidative stress level and mitochondrial function. Results: Resveratrol alleviated 6-hydroxy dopamine induced cell death through a dose dependent manner. Pre-treatment of resveratrol significantly relieved the reduction of DJ-1 level induced by 6-hydroxy dopamine, which contributed to a lower oxidative stress level and milder mito- chondrial injury. Conclusion: Pre-treatment of resveratrol protects cellular damage induced by 6-hydroxy dopamine. Moreover, it also alleviates cellular oxidative stress level and improves mitochondrial function. All these effects may due to promoting expression of DJ-1 protein induced by resveratrol. |
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