| 杨丽君,龙燕芬,詹望桃,石 莹,易丹妮.排卵期生殖道局部细胞免疫与黄体功能不全患者不孕的关系研究[J].,2017,17(10):1883-1885 |
| 排卵期生殖道局部细胞免疫与黄体功能不全患者不孕的关系研究 |
| Study on the Relationship between the Local Cellular Immunity in Genital Tract and Infertility in Patients with Luteal Phase Defect |
| 投稿时间：2016-09-13 修订日期：2016-10-10 |
| DOI：10.13241/j.cnki.pmb.2017.10.021 |
| 中文关键词: 排卵期 黄体功能不全 TNF-α IL-1β 局部细胞免疫 |
| 英文关键词: Ovulation Luteal phase defect TNF-α IL-1β Local cellular immunity |
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| 中文摘要: |
| 摘要 目的：探讨排卵期生殖道局部细胞免疫与黄体功能不全（LPD）患者不孕之间的关系，旨在为临床防治不孕不育提供依据及参考。方法：选择2014年10月~2015年10月本院确诊的LPD患者50例为观察组，同期筛选同期50例健康体检者为对照组，排卵后第4 d、6 d、8 d采用酶联免疫法检测两组排卵期血清孕酮（P）水平，采用放射免疫法（RIA）检测两组排卵期血清以及宫颈黏液中的肿瘤坏死因子-α（TNF-α）与白细胞介素1-β（IL-1β）的表达水平，对以上检测指标予以比较分析。结果：排卵后第4d、6d、8d观察组血清P明显低于对照组，比较差异均有统计学意义（P<0.05）。排卵期观察组血清TNF-α及IL-1β水平与对照组比较，差异不具有统计学意义（P>0.05），但观察组宫颈黏液中TNF-α及IL-1β水平显著高于对照组，差异有统计学意义（P<0.05）。排卵期观察组宫颈黏液中TNF-α、IL-1β水平与血清P存在负相关关系（r=-0.879，-0.886，P<0.05），且宫颈黏液中TNF-α、IL-1β二者存在显著正相关关系（r=0.768，P<0.05）。结论：排卵期生殖道局部细胞免疫激活，宫颈黏液中TNF-α及IL-1β水平升高，对黄体发育产生影响，并导致LPD的发生。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the relationship between the local cellular immunity in genital tract and infertility in patients with luteal phase defect (LPD), in order to provide the reference for prevention and treatment infertility. Methods: A total of 50 patients confirmed diagnosis with LPD were selected as the observation group from October 2014 to October 2015, and enrolled 50 healthy people as the control group in the same period, used enzymelinked immunosorbent assay to detect the levels of serum progesterone (P) in the two groups 4 d, 6 d, 8 d after ovulation, and used radioimmunoassay (RIA) method to detect the levels of cervical mucus and serum tumor necrosis factor-α (TNF-α) and interleukin-1-β (IL-1β) in two groups during ovulation, then above detection indexes were compared and analyzed. Results: Serum P levels in observation group were significantly lower than control group 4 d, 6 d, 8 d after ovulation respectively, the differences were statistically significant(P<0.05); The levels of erum TNF-α and IL-1β between the two groups was no significant difference during ovulation(P>0.05), while the levels of cervical mucus TNF-α and IL-1β in the observation group were significantly higher than control group during ovulation, the differences were statistically significant(P<0.05); The levels of cervical mucus TNF-α and IL-1β negatively correlated with serum P in the observation group during ovulation(r=-0.879, -0.886, P<0.05),moreover, the level of cervical mucus TNF-α positively correlated with cervical mucus IL-1β (r=0.768,P<0.05). Conclusion: Local cellular immune activation in genital tract during ovulation,with the levels of cervical mucus TNF-α and IL-1β increasing,which impact the corpus lutein and lead to the occurrence of LPD. |
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