| 姚 雷,孟令宇,常海钰,王丹阳,李 磊,梁玉梅.抑郁状态对患者调节性T细胞和调节性B细胞的作用研究[J].,2017,17(9):1716-1718 |
| 抑郁状态对患者调节性T细胞和调节性B细胞的作用研究 |
| Effects of Depression on Regulatory T Cells and Regulatory B Cells |
| 投稿时间：2016-09-23 修订日期：2016-10-19 |
| DOI：10.13241/j.cnki.pmb.2017.09.031 |
| 中文关键词: 抑郁 Treg细胞 Breg细胞 免疫 |
| 英文关键词: Depression Treg cell Breg cell Immunity |
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| 中文摘要: |
| 摘要 目的：探讨抑郁状态对患者调节性T细胞（Treg细胞）和调节性B细胞（Breg细胞）的作用。方法：收集入住我院40名抑郁患者，同时将来我院进行体检的40名健康志愿者作为对照组。分离各组的淋巴细胞，CD4、CD25和Foxp3标记Treg细胞，CD3、CD19和TGF-β标记Breg细胞，之后流式检测各组细胞的比例。结果：流式结果显示CD4+的辅助T细胞比例在抑郁组和对照组之间没有显著的差别（P＞0.05）；抑郁组中CD4+CD25+Foxp3+的Treg细胞的比例显著的高于对照组，升高了52.3%，结果具有统计学差异（P＜0.05）；CD3-CD19+的B细胞比例在抑郁组和对照组之间没有显著的差别（P＞0.05）；相对于对照组，抑郁组CD3-CD19+TGF-β+的Breg细胞比例显著升高，增加了91.7%，结果具有统计学差异（P＜0.05）。结论：抑郁患者体内Treg细胞和Breg细胞显著升高，引起机体免疫低下，我们的研究为了解神经和免疫的相关性提供了一定的理论参考。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of depression on the regulatory T cells (Treg cells) and regulatory B cells (Breg cells). Methods: We collected 40 patients with depression in our hospital, and 40 healthy volunteers were served as control group. Lym- phocytes were separated, CD4, CD25 and Foxp3 antibodies were used to label Treg cells; CD3, CD19, and TGF-β antibodies were used to label Breg cells, then flow cytometry was used to detect cell proportion in each group. Results: Flow cytometry results showed CD4+ T cells had no significant difference between depression and the control group (P>0.05), and The proportion of CD4+CD25+Foxp3+ Treg cells in the depression group was significantly higher than that in the control group, which increased 52.3% and the results were statisti- cally significant (P<0.05); CD3-CD19+ Breg cells had no significant difference between depression and the control group (P>0.05); Compared with the control group, the proportion of CD3-CD19+TGF-β+ Breg cells in depression group was significantly higher than that in the control group, which increased by 91.7%, and the results were statistically significant (P<0.05). Conclusion: Treg cells and Breg cells were significantly increased in depression patients, causing low immunity, our study provides a theoretical reference for under- standing the correlation between nerve and immune. |
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