| 袁丽粉,乔建瓯,孙淑宁,郑翠侠,王 健.前列腺素D2在小鼠哮喘模型中表达水平的研究[J].,2017,17(9):1606-1609 |
| 前列腺素D2在小鼠哮喘模型中表达水平的研究 |
| Study on the Expression Level of Prostaglandin D2 in Mouse Asthma Model |
| 投稿时间：2016-09-08 修订日期：2016-09-30 |
| DOI：10.13241/j.cnki.pmb.2017.09.002 |
| 中文关键词: 哮喘 前列腺素D2 卵白蛋白 嗜酸性粒细胞计数 |
| 英文关键词: Asthma Prostaglandin D2 Ovalbumin Eosinophil count |
| 基金项目:国家自然科学基金项目（81170028）；上海交通大学医学院科技基金项目(12XJ10020) |
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| 中文摘要: |
| 摘要 目的：探讨前列腺素D2在小鼠哮喘模型中的表达水平。方法：24只小鼠随机均分为四组：正常对照2周组，正常对照4周组，哮喘模型2周组，哮喘模型4周组。以卵白蛋白和氢氧化铝混悬液致敏，并予以卵白蛋白雾化激发建立哮喘小鼠模型。末次激发24h后，ELISA法检测其血清及支气管肺泡灌洗液（bronchoalveolar lavage fluid,BALF）中前列腺素D2水平；收集BALF行嗜酸性粒细胞（eosinophil，EOS）计数；HE染色观察其肺组织病理改变。结果：与正常对照2周及4周组相比，哮喘模型组小鼠肺组织病理损害明显加重，EOS计数哮喘组显著高于正常组，差异有统计学意义（P<0.05），血清及BALF中的前列腺素D2水平明显高于正常组，差异有统计学意义（P<0.01），而且随着哮喘病程的延长，即哮喘模型4周组较2周组的肺组织病理损害更为严重，EOS计数升高更为显著，血清及BALF中的前列腺素D2增多更为明显，差异有统计学意义（P<0.05）。但正常对照4周组与2周组比较，未见明显变化。结论：炎性介质前列腺素D2水平在哮喘模型中有一定的表达水平，而且随着哮喘病程的延长，前列腺素D2表达水平也逐渐升高。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression level of prostaglandin D2 in mouse asthma model. Methods: 24 mice were randomly divided into four groups: normal control group for 2 weeks, normal control group for 4 weeks, asthma model group for 2 weeks and asthma model group for 4 weeks. The mice in asthma model group were sensitized by Ovalbumin (OVA) and aluminum hydroxide (Al(OH)3) suspension and be aerosolized by ova. At the end, the levels of PGD2 in the serum and bronchoalveolar lavage fluid（BALF）samples were detected through ELISA, and the eosinophils of the BALF were counted, and Hematoxylin and eosin (HE) staining was performed to observe the lung tissue pathological changes. Results: Compared with the normal groups, the pathological damage of lung tissues in asthma model group was significantly increased. The eosinophils counting of BALF in asthma group were significantly lower than those in normal group, and the difference was statistically significant (P<0.05). The PGD2 levels in serum and BALF were signifi- cantly higher than that in the normal groups, the difference was statistically significant (P<0.01). But, along with the prolonging of dura- tion of asthma, lung tissue damage of asthma model in 4 weeks group was more serious than that of 2 weeks group, and EOS counts were higher in serum and BALF, prostaglandin D2 was increased more significantly, and the difference was statistically significant (P<0.05). But, there was no obvious change between the two normal control groups. Conclusion: The inflammatory mediator, PGD2, has a certain level of expression in the asthma model, and PGD2 level is gradually increased with the prolongation of the duration of asthma. |
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