| 王彩 高培鑫 许竞男 于文功 路新枝△.小鼠粪便中短链脂肪酸提取与检测方法的建立及应用[J].,2017,17(6):1012-1015 |
| 小鼠粪便中短链脂肪酸提取与检测方法的建立及应用 |
| Establishment and Application of Extraction and Determination Method forShort-Chain Fatty Acids in Feces of Mice |
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| DOI： |
| 中文关键词: 短链脂肪酸 高效液相色谱法 液-液萃取 新琼寡糖 |
| 英文关键词: SCFAs HPLC Liquid-liquid extraction Neoagaro-oligosaccharides |
| 基金项目:国家自然科学基金项目（41376144）；国家科技支撑计划（2013BAB01B02）；山东省科技发展计划（2014GGH215002） |
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| 中文摘要: |
| 目的：建立小鼠粪便中三种短链脂肪酸（乙酸、丙酸、丁酸）的检测方法及其应用。方法：使用水提法和乙醚萃取法提取小鼠粪
便中的短链脂肪酸（SCFA），通过高效液相色谱（HPLC）评价两种方法的提取效果，并建立短链脂肪酸的HPLC 定量检测方法并
进行方法学考察，利用上述建立的体系评价新琼寡糖对小鼠短链脂肪酸合成的影响。结果：确定了SCFA 的乙醚萃取方法和基于
HPLC 的定量检测方法，被测组分浓度与其峰面积呈良好线性关系，各组分测定的相对标准偏差在1.31%～2.61%之间，平均加标
回收率为86.6%～105.8%之间；对喂饲新琼寡糖小鼠粪便中的SCFA 检测显示，较对照组乙酸和丙酸含量提高了1 倍，丁酸含量
提高了0.45 倍。结论：建立了小鼠粪便中SCFA 的提取和定量检测方法，并利用该方法证明了新琼寡糖能促进小鼠短链脂肪酸的
合成。 |
| 英文摘要: |
| Objective:A rapid and sensitive method was developed and applied for the extraction and determination of three
short-chain fatty acid (SCFA) in feces.Methods:SCFAs were purified by aqueous or diethyl ether extraction of feces. HPLC was used to
evaluate the purification method and to quantitative analyze of SCFA. At last, the method was applied to investigate the effects of
neoagaro-oligosaccharides(NAOS) on SCFA production in mice.Results:Diethyl ether extraction was proved to be better for purifying of
SCFA from feces. The detection results showed that acetic acid, propionic acid and n-butyric acid all had a good liner relationship, the
standard deviation was 1.31%～2.61%, and the recovery rate was 86.6%～105.8%. After feeding of NAOS for 7 days, the feces were
collected to monitor the amount of SCFA, and the results demonstrated that the concentrations of acetic acid and propionic acid were
increased for1-fold by comparing with the control group, and n-butyric was increased for 0.45-fold.Conclusion:The method for
extraction and quantification of SCFAs was established, and NAOS was proved can improve the synthesis of SCFAs in mice. |
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