| 栗颖利 邢新 杨超 戴海英 方硕 唐炜雅.脂肪干细胞与放射后成纤维细胞共培养模型的建立和lncRNA高通量测序[J].,2017,17(6):1006-1011 |
| 脂肪干细胞与放射后成纤维细胞共培养模型的建立和lncRNA高通量测序 |
| A Co-culture Model of Adipose Derived StemCells and Fibroblasts after Irradiation and High Throughput Sequencing of lncRNA |
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| DOI： |
| 中文关键词: 脂肪干细胞 成纤维细胞 共培养 测序 |
| 英文关键词: Adipose derived stemcells Fibroblasts Co-culture Sequencing |
| 基金项目:国家自然科学基金项目(81301644)；国家自然科学基金项目(81272119) |
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| 中文摘要: |
| 目的：探讨大鼠脂肪来源干细胞(adipose-derived stemcells，ADSCs)对放射后成纤维细胞(fibroblast，Fb)的影响。方法：体外原
代培养SD大鼠ADSCs 和真皮Fb，分别对两种细胞进行鉴定，Fb 辐照后两种细胞建立共培养模型。将Fb分为3 组，对其进行
lncRNA 和mRNA转录本高通量测序。结果：ADSCs 高表达CD29 和CD44，低表达CD31 和CD45。大鼠Fb 高表达波形蛋白。Fb
单纯辐照和干细胞干预后转录水平有明显的差异。结论：ADSCs 能使辐射后的Fb 细胞lncRNA 和mRNA转录水平明显的改变，
提示多项生物学功能及信号通路在其中发挥作用。 |
| 英文摘要: |
| Objective:To discuss the effect of rat adipose derived stem cells (ADSCs) on fibroblasts (Fb) after radiation.Methods:We cultivated ADSCs and dermal Fb of SD rats in vitro, and two kinds of cells were identified. Then a co-culture model was established.
Fb was divided into 3 groups, which carry on lncRNA and mRNA high throughput sequencing.Results:The high expression of CD29
and CD44 were identified in ADSCs, and the low expression of CD31 and CD45 in Fb. There was a significant difference in the level of
transcripts between control group and Fb group of ADSCs Intervention after radiation.Conclusion:ADSCs can significantly alter the
lncRNA and mRNA transcription levels of Fb cells after irradiation, suggesting that a number of biological functions and signaling
pathways play an important role in the prcess. |
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